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Key Documents

CBL555

Sigma-Aldrich

Anti-CD34 Class III Antibody, clone 581

clone 581, Chemicon®, from mouse

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

581, monoclonal

Espèces réactives

human

Fabricant/nom de marque

Chemicon®

Technique(s)

flow cytometry: suitable

Isotype

IgG1

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... CD34(947)

Spécificité

Clone 581 reacts with the class III CD34 epitope is resistant to neuraminidase, chymopapain and glycoprotease.

The antibody reacts with a single-chain 105-120 kDa heavily O-glycosylated transmembrane glycoprotein which is expressed on haematopoietic progenitor cells, vascular endothelium and some tissue fibroblasts. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. CD34 may also play a role in the adhesion of specific antigens to endothelium.

Application

Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Study of the Class III CD34 epitope by FACS

Optimal working dilutions must be determined by the end user.
This Anti-CD34 Antibody, Class III, clone 581 is validated for use in FC for the detection of CD34 Class III.

Forme physique

Format: Purified
Protein G affinity chromatography. The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Stockage et stabilité

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Positive selection of bone marrow-derived CD34 positive cells for possible stem cell transplantation.
Egeland, T, et al.
Transplantation Proceedings, 25, 1261-1263 (1993)
Anthony Zulli et al.
Experimental physiology, 93(5), 564-569 (2008-01-15)
Angiotensin-converting enzyme 2 (ACE2) is a novel enzyme with possible implications in the treatment of blood pressure disorders. Recent evidence suggests that an upregulation of ACE2 can be stimulated by all-trans retinoic acid (at-RA); however, at-RA also affects regulation of
Teresa Massam-Wu et al.
Methods in molecular biology (Clifton, N.J.), 1722, 249-260 (2017-12-22)
We present a method to capture mesenchymal stromal cells (MSCs) by adhesion to extracellular matrix (ECM) molecules under flow conditions. The technique simulates a physiological system and exploits the natural biological interactions of cells, through integrin receptors, with their ECM.
Mahmoud Yousefifard et al.
Journal of stem cells & regenerative medicine, 18(2), 53-63 (2023-01-31)
Few studies are conducted on the efficacy of human adipose-derived stem cells (ADSCs) in spinal cord injury (SCI) management and electrophysiological changes in the spinal cord. Therefore, the present study aimed to determine the effect of ADSCs on neuropathic pain

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