Skip to Content
Merck

HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18

HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

53829-U

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

standard

Product No.
Description
Pricing

F7876

Folic acid

≥97%

M9929

Methotrexate

meets USP testing specifications

CONDITIONS

column

Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)

mobile phase

[A] 10 mM ammonium formate, pH 3.0; [B] acetonitrile; (90:10, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 280 nm

injection

5 μL

Description

Analysis Note

This application demonstrates the suitability of the Ascentis C18 for the analysis of aminopterin, folic acid and methotrexate. Structures along with the optimized chromatogram obtained on the Ascentis Express C18 are presented

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS