- Sulfonyl fluoride analogues as activity-based probes for serine proteases.
Sulfonyl fluoride analogues as activity-based probes for serine proteases.
Chembiochem : a European journal of chemical biology (2012-09-26)
D Alexander Shannon, Christian Gu, Christopher J McLaughlin, Markus Kaiser, Renier A L van der Hoorn, Eranthie Weerapana
PMID23008217
ABSTRACT
Enriched with fluoride: To expand on the available tools to interrogate proteases, we explored sulfonyl fluorides as activity-based probes. An alkyne-tagged sulfonyl fluoride covalently modifies members of the S1 family of serine proteases. By applying click chemistry, avidin enrichment and mass spectrometry, we can enrich and identify active endogenous serine proteases from a complex proteome.
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