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usage
sufficient for 100 tests
Quality Level
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
technique(s)
flow cytometry: suitable
input
sample type cultured cells
detection method
fluorometric
shipped in
dry ice
storage temp.
−20°C
General description
A convenient and sensitive method for the detection of activated caspase-3 in living cells. The fluorescent marker, FITC-DEVD-FMK, is a cell-permeable, non-toxic inhibitor that binds irreversibly to activated caspase-3 in apoptotic cells. The fluorescence intensity can be measured by fluorescence microscopy, fluorescence plate reader, or flow cytometry.
Specificity
A broad range of species
Packaging
1 kit in Glass bottle
Components
Labeled Caspase-3 Inhibitor (FITC-DEVD-FMK), Wash Buffer, Caspase-3 Inhibitor (Z-VAD-FMK), and a user protocol.
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS
Specifications
Assay Time: 1.5 h
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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