Skip to Content
Merck
All Photos(1)

Documents

M2909

Sigma-Aldrich

Murashige and Skoog Modified Basal Salt Mixture

powder, suitable for plant cell culture

Synonym(s):

Murashige and Skoog Modified Basal Salt Mixture, w/o Ammonium Nitrate

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352207
NACRES:
NA.72

form

powder

Quality Level

200

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

Related Categories

Application

Murashige and Skoog Modified Basal Salt Mixture has been used:
  • to promote root development for internode stem suckers
  • to promote shoot growth for the calluses
  • for low nitrogen (LN) treatment, for the growth of Arabidopsis thaliana

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Formula variant

Without NH4NO3
With the macro- and micronutrients described by Murashige and Skoog (1962).

Media Formulation

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M2909) contain the macronutrients and micronutrients of the original classic formulation without ammonium nitrate. It can be combined with M&S vitamins or Gamborg′s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Pictograms

Flame over circleExclamation mark
GHS03,GHS07

Signal Word

Warning

Hazard Statements

H272 - H319

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 3

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH SVHC Candidate List

CAS No.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Iron homeostasis alteration in transgenic tobacco overexpressing ferritin
Van WO, et al.
The Plant Journal, 17(1), 93-97 (1999)
Callus induction and plant regeneration from mature embryos of a diverse set of wheat genotypes
Zale JM, et al.
Plant Cell, Tissue and Organ Culture, 76(3), 277-281 (2004)
Interactions of abscisic acid and sugar signalling in the regulation of leaf senescence
Pourtau N, et al.
Planta, 219(5), 765-772 (2004)
Gabriele B Monshausen et al.
The Plant cell, 21(8), 2341-2356 (2009-08-06)
Mechanical stimulation of plants triggers a cytoplasmic Ca(2+) increase that is thought to link the touch stimulus to appropriate growth responses. We found that in roots of Arabidopsis thaliana, external and endogenously generated mechanical forces consistently trigger rapid and transient
O Van Wuytswinkel et al.
The Plant journal : for cell and molecular biology, 17(1), 93-97 (1999-03-09)
Intracellular iron concentration requires tight control and is regulated both at the uptake and storage levels. Our knowledge of the role that the iron-storage protein ferritins play in plants is still very limited. Overexpression of this protein, either in the

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service