A8582

Antifoam SE-15

aqueous emulsion for bacterial and mammalian systems

• Synonym(s): silicone-based antifoam
• UNSPSC Code: 12161901
• NACRES: NA.85

Properties

• Quality Level: 200
• form: emulsion (aqueous)
• concentration: 10% (active silicon)
• technique(s): cell culture | hybridoma: suitable
• solubility: H₂O: soluble (effective hot or cold.)
• density: 1.0 g/mL at 25 °C
• application(s): microbiology
• storage temp.: room temp
• suitability: (Mammalian (suspension)); bacteria (fermentation)

Description

General description

Antifoam SE-15 is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.

Application

Antifoam SE-15 has been used:

• in the preparation of ramified rolling circle amplification (RAM) amplification buffer.
• to find its effects on cell viability, virus infectivity and PCR (polymerase chain reaction) performance.
• as a constituent of phosphate-buffered saline (PBS) for the determination of total antioxidant status (TAOS) by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay.
• as a component of autoinduction expression culture.
• as a component of cyclic adenosine monophosphate (cAMP) detection buffer.

Features and Benefits

• Controls foaming in microbial culture flasks or fermentations
• Non-toxic to the culture medium
• Can be pumped to fermenter on an as-needed basis

Components

Antifoam SE-15 is an aqueous emulsion containing 10% active silicon and non-ionic emulsifiers .

Preparation Note

Antifoam SE-15 can be pre-diluted with 3-10 parts of cool water to aid in dispersion. Pre-diluted
suspensions should be used immediately.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Warning
• Hazard Statements: H319,H373
• Precautionary Statements: P260 - P264 - P280 - P305 + P351 + P338 - P314 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - STOT RE 2 Inhalation
• Target Organs: Lungs
• WGK: WGK 1