ABT65

Anti-HAX-1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): HCLS1-associated protein X-1, HS1-associating protein X-1, HAX-1, HS1-binding protein 1, HSP1BP-1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, rat, human
• technique(s): immunocytochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_006109
• UniProt accession no.: O00165
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... HAX1(10456)

Description

General description

HAX-1 (HS1-associated protein X-1) is a mitochondrial anti-apoptotic protein and is thought to be regulated by Omi/HtrA2. HAX-1 has been found to associate with BSEP, MDR1 and MDR2 ABC-transporters and interacts with cortactin to play a role in BSEP internalization from the apical membrane. HAX-1 and alphavbeta6 are found to be highly expressed in certain oral cancers and work together to enhance tumor invasion and progression. High expression of HAX-1 in many other cancer tissues shows that HAX-1 is important in neoplastic transformation.

Specificity

This antibody recognizes HAX-1 at the domains involved in HCLS1-, CASP9-, and GNA13-binding.

Immunogen

Epitope: HCLS1-, CASP9-, and GNA13-binding domains

KLH-conjugated linear peptide corresponding to the domain of human HAX-1 that is involved in HCLS1-, CASP9-, and GNA13-bindings.

Application

Anti-HAX-1 Antibody is an antibody against HAX-1 for use in WB & IC.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Cytoskeleton

Western Blot Analysis: 0.1 µg/mL from a representative lot detected HAX-1 in 10 µg of HeLa cell lysate and rat liver tissue lysate.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected HAX-1 in NIH/3T3 and HeLa cells.

Quality

Evaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected HAX-1 in 10 µg of Jurkat cell lysate.

Target description

~35 kDa observed.
HAX-1 has been shown to migrate as a ~34-36 kDa band in WB (Ortiz, D.F., et al. (2004). JBC. 279(31):32761-32770).

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Jurkat cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable