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HPLC Analysis of Warfarin Enantiomers on Astec® CHIROBIOTIC® V2

HPLC Analysis of Warfarin Enantiomers on Astec® CHIROBIOTIC® V2 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Astec® CHIROBIOTIC® V2 Chiral HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

standard

Product No.
Description
Pricing

Warfarin

analytical standard

Warfarin

PESTANAL®, analytical standard

(R)-(+)-Warfarin

≥97% (HPLC)

(S)-(−)-Warfarin

≥97% (HPLC)

CONDITIONS

column

CHIROBIOTIC V2, 25 cm x 4.6 mm I.D., 5 μm particles (15024AST)

mobile phase

[A] 10 mM ammonium acetate, pH 4.1; [B] acetonitrile; (70:30, A:B)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 280 nm

injection

10 μL

sample

1 mg/mL in methanol

Description

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC
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