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HPLC Analysis of 16 Triazine and Phenyl Urea Compounds on Ascentis® Express RP-Amide

HPLC Analysis of 16 Triazine and Phenyl Urea Compounds on Ascentis® Express RP-Amide application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® Express RP-Amide, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 3 mm

standard

Product No.
Description
Pricing

Atrazine

PESTANAL®, analytical standard

Terbumeton-desethyl

PESTANAL®, analytical standard

Atrazine-desisopropyl

PESTANAL®, analytical standard

Isoproturon

PESTANAL®, analytical standard

Linuron

PESTANAL®, analytical standard

Diuron

PESTANAL®, analytical standard

Chlortoluron

PESTANAL®, analytical standard

Cyanazine

PESTANAL®, analytical standard

Terbuthylazin-desethyl

PESTANAL®, analytical standard

Carbendazim

analytical standard

CONDITIONS

column

Ascentis Express RP-Amide, 15 cm x 3 mm I.D., 2.7 μm particles (53919-U)

mobile phase

[A] water; [B] acetonitrile

gradient

5 to 53% B in 70 min

flow rate

0.4 mL/min

column temp.

35 °C

detector

UV, 240 nm

injection

5 μL

sample

20 mg/L (except terbuthylazin desethyl and terbuthylazine 60 mg/L) in 60:40, water: acetonitrile

Description

Analysis Note

This application demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of 16 triazine and phenyl urea compounds. Lower column temperature can improve resolution of the critical pair.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany