The effect of Ti anodized nano-foveolae structure on preosteoblast growth and osteogenic gene expression.

The effect of Ti anodized nano-foveolae structure on preosteoblast growth and osteogenic gene expression.

Journal of nanoscience and nanotechnology (2014-04-18)

Wei-Qiang Yu, Ling Xu, Fu-Qiang Zhang

ABSTRACT

The TiO2 nanotubes by anodization have been extensively studied for medical implant and orthopedic applications because of enhancing bone development. In the present study, a new nano-foveolae structure verified by SEM and AFM was prepared by simulating the nanotubes exfoliation from anodized Ti. MC3T3-E1 preosteoblasts were used to investigate the effects of new nanoscale surface on cell spreading, proliferation, and osteogenic gene expression. The new nano-foveolae structure supported preosteoblast better spreading, more filopodiaes shown by SEM (4 h), and lower proliferation (72 h) than the smooth Ti. By two weeks, the new nanoscale surface induced higher expression of osteogenic markers alkaline phosphatase (ALP), osteopontin (OPN), osteocalcin (OCN), and collagen I (COL I) with real-time RT-PCR compared to the control group. The results of this study suggest that the new nano-foveolae structure provides a favorable surface for functions of bone cells.

MATERIALS

Product Number

Brand

Product Description

IRMM531A

Titanium, IRMM^®, certified reference material, 0.1 mm foil

348864

Sigma-Aldrich

Titanium, wire, diam. 0.5 mm, 99.99% trace metals basis

348805

Sigma-Aldrich

Titanium, foil, thickness 0.5 mm, 99.99% trace metals basis

268496

Sigma-Aldrich

Titanium, powder, −100 mesh, 99.7% trace metals basis

366994

Sigma-Aldrich

Titanium, powder, <45 μm avg. part. size, 99.98% trace metals basis

433667

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268526

Sigma-Aldrich

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460397

Sigma-Aldrich

Titanium, foil, thickness 0.127 mm, ≥99.99% trace metals basis

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Titanium, rod, 100mm, diameter 4mm, annealed, 99.6+%

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Titanium, rod, 100mm, diameter 16mm, annealed, 99.6+%

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Titanium, rod, 50mm, diameter 9.5mm, annealed, 99.99+%

GF35130035

Titanium, rod, 25mm, diameter 9.5mm, annealed, 99.99+%

GF60092456

Titanium, tube, 1000mm, outside diameter 0.81mm, inside diameter 0.61mm, wall thickness 0.1mm, as drawn, 99.6+%

GF83238127

Titanium, tube, 100mm, outside diameter 3.0mm, inside diameter 1.5mm, wall thickness 0.75mm, annealed, 99.6+%

GF06003100

Titanium, tube, 100mm, outside diameter 9.5mm, inside diameter 8.2mm, wall thickness 0.65mm, annealed, 99.6+%

GF20610997

Titanium, tube, 1000mm, outside diameter 6.1mm, inside diameter 5.1mm, wall thickness 0.5mm, annealed, 99.6+%

GF21200746

Titanium, tube, 100mm, outside diameter 0.81mm, inside diameter 0.61mm, wall thickness 0.1mm, as drawn, 99.6+%

GF99390134

Titanium, rod, 100mm, diameter 3.0mm, annealed, 99.6+%

GF73866998

Titanium, tube, 100mm, outside diameter 12.7mm, inside diameter 10.9mm, wall thickness 0.9mm, annealed, 99.6+%

GF86068536

Titanium, rod, 1000mm, diameter 2mm, annealed, 99.6+%

GF17395375

Titanium, tube, 1000mm, outside diameter 2.03mm, inside diameter 1.63mm, wall thickness 0.2mm, annealed, 99.6+%

GF45586985

Titanium, rod, 200mm, diameter 1.5mm, annealed, 99.6+%

GF75709457

Titanium, sphere, 100pcs, diameter 3mm, 99.6+%

GF66495279

Titanium, tube, 1000mm, outside diameter 3.0mm, inside diameter 1.5mm, wall thickness 0.75mm, annealed, 99.6+%

GF38954076

Titanium, rod, 200mm, diameter 6mm, annealed, 99.6+%

GF01312490

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GF48092687

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