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[Modification of the method of preparation of paraffin blocks].

[Modification of the method of preparation of paraffin blocks].

Arkhiv patologii (1984-01-01)

V G Shlopov, N A Krivenko, T I Shevchenko

ABSTRACT

The quality of paraffin sections is determined to a considerable degree by the properties of paraffin block such as crystal size and spatial homogeneity. A new temperature regimen of treatment in preparation of paraffin blocks has been developed. After impregnation, pieces of tissue are overlayed with paraffin at a temperature of 56-60 degrees C in paper-tin foil dishes and then immediately placed in an antifreeze at 0 degree to -5 degrees C. The internal stresses, deformations, and microcracks occurring in this procedure are eliminated by incubation of the resulting block in a thermostat at 45 degrees C in vapours of low-melting fraction of paraffin (2-24 hours) followed by rapid cooling with cold air (from -5 degrees to -10 degrees C). The second step of the treatment may also be used for improvement of the quality of paraffin blocks after long-term storage.

MATERIALS

Product Number

Brand

Product Description

Tin, foil, not light tested, 150x150mm, thickness 0.009mm, 97.4%

Tin, foil, not light tested, 25x25mm, thickness 0.006mm, 99.75%

Tin, foil, light tested, 50x50mm, thickness 0.008mm, 99.75%

Tin, foil, not light tested, 150x150mm, thickness 0.007mm, 97.4%

Tin, foil, not light tested, 150x150mm, thickness 0.006mm, 97.4%

Tin, foil, 10m coil, thickness 0.084mm, coil width 41mm, as rolled, 99.95%

Tin, foil, 10m coil, thickness 0.009mm, 97.4%

Tin, foil, 25mm disks, thickness 0.06mm, as rolled, 99.99+%

Tin, foil, 4mm disks, thickness 0.0125mm, 97.4%

Tin, foil, 20m coil, thickness 0.096mm, coil width 41mm, as rolled, 99.95%

Tin, foil, 50mm disks, thickness 0.25mm, as rolled, 99.99+%

Tin, foil, 4mm disks, thickness 0.015mm, 99.75%

Tin, foil, 2m coil, thickness 0.0125mm, 97.4%

Tin, foil, 10mm disks, thickness 0.025mm, as rolled, 98.8%

Tin, foil, 10mm disks, thickness 1.0mm, as rolled, 100%

Tin, foil, 10mm disks, thickness 0.02mm, 99.75%

Tin, foil, 25mm disks, thickness 0.5mm, as rolled, 98.8%

Tin, foil, 25mm disks, thickness 0.01mm, 97.4%

Tin, foil, 4mm disks, thickness 0.05mm, as rolled, 98.8%

Tin, foil, 25mm disks, thickness 0.025mm, as rolled, 99.99+%

Tin, foil, 5m coil, thickness 0.025mm, as rolled, 98.8%

Tin, foil, 2m coil, thickness 0.072mm, coil width 41mm, as rolled, 99.95%

Tin, foil, 2m coil, thickness 0.084mm, coil width 41mm, as rolled, 99.95%

Tin, foil, 50mm disks, thickness 0.025mm, as rolled, 98.8%

Tin, foil, 5m coil, thickness 0.1mm, as rolled, 98.8%

Tin, foil, 6mm disks, thickness 0.009mm, 97.4%

Tin, foil, 8mm disks, thickness 0.030mm, as rolled, 97.4%

Tin, foil, 6mm disks, thickness 0.006mm, 97.4%

Tin, foil, 50mm disks, thickness 1.0mm, as rolled, 99.99+%

Tin, foil, 4mm disks, thickness 0.20mm, as rolled, 98.8%