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Millicell® Cell Culture Inserts & Plates

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Millicell® hanging inserts for cell culture, co-culture, and tissue modeling

Millicell® hanging cell culture inserts

Millicell® cultureware for biologically relevant results

Millicell® standing cell culture inserts

Millicell<sup>®</sup> HY 3-layer cell culture flask, T-600, sterile Millicell<sup>®</sup> HY 3-layer flask, T-600, sterile high yield cell culture flasks provide a consistent, quality growth environment across all layers, with the same volume of media in each layer & the same media requirements as traditional T-flasks.

Millicell® HY high yield flask

Millicell<sup>®</sup>-96 Cell Culture Insert Plate

Millicell®-96 Cell Culture Insert Plate

Millicell<sup>®</sup>-24 Cell Culture Insert Plate

Millicell®-24 Cell Culture Insert Plate

Millicell® EZ slides for all-in-one culturing, fixing, staining, and viewing of cells

Millicell® EZ Slides

Millicell® cell culture inserts are designed to maximize biological relevance and user convenience in cell culture applications. In vivo, cells live in a three-dimensional environment and can access nutrients from all directions. In contrast, traditional plastic culture plates force cells to grow on a smooth, two-dimensional surface, leading to flattened nuclei and poor function. Millicell® permeable inserts and plates feature a design that allows access to both the basolateral and apical surfaces of cell layers. This promotes natural, multidimensional growth and provides more relevant in vitro models, leading to more biologically relevant results for applications including toxicity screening, primary and secondary screening, cell signaling, cell proliferation, transport assays, and ADME/Tox drug safety studies.

POPULAR CATEGORIES

Image of Flask
Hanging Inserts
Standing Inserts
Standing Inserts
Plate Assemblies
Plate Assemblies
Chamber Slides
Chamber Slides
Multilayer Flasks
Multilayer Flasks
Cell culturing with Millicell<sup>®</sup> hanging inserts

Cell culture with Millicell® hanging inserts

Millicell® Hanging Inserts for Cell Culture

Millicell® hanging inserts make it possible to study both apical and basal surfaces of the cell monolayer, and are excellent tools for co-culture, as there is no disturbance of the outer basolateral cell culture by the presence of insert feet.

  • For 2.5D cell culture and chemotaxis
  • Unique design facilitates basolateral access and reduces risk of contamination
  • Optically clear PET membrane allows for enhanced cell visualization and monitoring of the cell monolayer
  • High pore density translucent PET membrane for increased rates of basolateral diffusion of nutrients
  • Available in three well sizes (6, 12 and 24-well) and five membrane pore sizes (0.4 µm to 8.0 µm) 
  • Compatible with fluorophores for fluorescent imaging

Millicell® Standing Inserts for Cell Culture

Millicell® Standing Inserts are free-standing inserts that can rest at the bottom of cell culture plate wells and other vessels, elevated by insert feet. These standing inserts promote the natural growth of suspension and adherent cell lines and are available in three different optimized membranes tailored for specific needs.

  • Biopore™ PTFE (polytetrafluoroethylene) membranes are low protein-binding membranes developed for live cell viewing and immunofluorescent applications. The transparent membrane exhibits low background fluorescence compared to other membrane materials and is suitable for attachment-dependent cells when coated with an extracellular matrix.
  • MF-Millipore™ MCE (mixed cellulose esters) membranes are Triton®-free and can be used for cell surface receptor, in vitro toxicology, microbial attachment, and polarized uptake assays.
  • Isopore™ PCF (polycarbonate) membranes are hydrophilic and tissue culture-treated to allow growth of adherent phenotype cells without the use of extracellular coating matrix (ECM). The high pore density allows for maximum diffusion in drug transport and uptake assays.

Millicell® Organotypic Standing Inserts are designed for superior study of three-dimensional explant structures.

  • For high cell viability and superior study of three-dimensional explant structures
  • Shorter profile allows inserts to fit inside a standard petri dish
  • Optically clear Biopore™ (PTFE) membrane supports high viability and excellent trans-membrane oxygen transport, and is optimized for long-term explant maintenance

Millicell® Plate Assemblies

Millicell®-24 and Millicell®-96 Plate Assemblies enable cell growth, feeding, and analysis in one membrane-bottom plate optimized to grow and sustain high-integrity cell monolayers. These plates can be used manually or with automated cell seeding, feeding, and washing systems. Their thoughtful design improves function with seed-and-feed systems, automated liquid handling systems, and transepithelial electrical resistance (TEER) measurement instruments.

  • Complete system with a multi-well membrane-bottom plate, single-well or multi-well receiver tray, and lid
  • Apical assist protects the cell monolayer and allows for easier pipetting and basolateral access
  • Available in a variety of polyethylene terephthalate (PET) membrane pore sizes.
  • Teardrop-shaped receiver wells eliminate air bubbles
  • Raised well edges for improved tape sealing and large font labeling for easy well identification
Millicell® Ultra-low Attachment 96-well plate

Millicell® Ultra-Low Attachment (ULA) Plates and Millicell® Microwell Plates

Millicell® ULA plates are reliable 3D cell culture tools that promote scaffold free self-assembly of spheroids. The 96-well U bottom plates are compatible with analysis and brightfield and fluorescence imaging as the wells have high optical clarity. The non-cytotoxic, stable, and non-adhesion surface of Millicell® ULA plates support high throughput spheroid assembly.

  • Promote uniform single spheroid/EB formation in each well
  • Spheroid formation and analysis in the same plate
  • Pre-coated with a non-binding surface for cells to facilitate natural spheroid formation
  • Plates are compatible with liquid robotic system
Millicell® Microwell 96-well plate showing the microwells within the wells in the plate

Millicell® Microwell plates are ready-to-use 3D culture tools for high throughput and reproducible organoid culture. Based on an array of ultra-dense U-bottom shaped microwells (μW), single organoids are generated in each microcavity and grown in a pre-cast hydrogel without a solid extracellular matrix. With multiple configurations and microwell diameters available, Millicell® Microwell plates fit your organoid application needs.

  • Plates contain polyethylene glycol (PEG) hydrogel in the microwells
  • Homogeneous organoids form in one focal plane for high throughput imaging
  • Up to hundreds of microwells in a single well scale up organoid generation for high throughput applications
  • SBS standard plate format for easy implementation of standardized organoid culture, screening applications, and automation workflows.

How to select a cell culture insert

Recommended membrane material and pore sizes for Millicell® cell culture inserts and plates are based on cell type and application.

  • CM = Biopore™ hydrophilic PTFE membrane
  • HA = MF-Millipore™ mixed cellulose esters membrane
  • PCF = Isopore™ polycarbonate membrane
  • PET = PET (polyethylene terephthalate)
Table 1. Millicell® insert and plate membrane type, pore size, and applications.

Millicell® EZ Slides

Millicell® EZ Slides can be used to culture, fix, stain and view your cells all in one device. There’s no need to tediously move cover slips from your culture dish to a slide, or to remove the media chamber from the slide prior to fixing or staining. Easily remove the chamber structure without worrying about breaking slides or ruining cell layers. Acquire data simply and quickly with Millicell® EZ Slides.

  • Breakable tabs for easy well removal
  • No tools required to remove wells from slide
  • No adhesive used to attach wells to slide
  • No leaky wells
  • Polypropylene wells facilitate fixing and staining with wells still attached, allowing different probes in wells
  • Slide holder protects slide from damage

Millicell® HY Flasks

Millicell® HY Flasks are innovative multilayer cell culture flasks that support uniform, consistent cell growth for high cell recovery. Manufactured with multiple identical layers, these flasks maximize cell culture surface area for the highest yields, while saving incubator space.

  • Save time spent passaging cells
  • Save costs associated with media, cells, and reagents
  • Consistently high cell yields
  • Uniform health across all layers
  • Easy transition from single-layer cell culture flasks
  • Fits under microscopes for monitoring cell health and confluency
  • Stackable design saves incubator space

Related Product Resources

Article: Alternative High-Yield Cell Culture Systems
Brochure: Multiply Your Success MultiScreen® and Millicell® Filter Plates for Assay Development
Data Sheet: Millicell® Inserts and Plates
Flyer: Discover a Better Cell Culture Insert
Generating Organotypic 3D Skin Cultures as an In-Vitro Model of the Human Epidermis
Protocol: T-Cell Migration Assays Using Millicell® Cell Culture Inserts
Technical Article: Barrier Formation and Permeability Assays using Millicell® Hanging Cell Culture Inserts
Technical Article: Cell Migration and Invasion Assay Guidance using Millicell® Cell Culture Inserts
Using Immortalized 16HBE14o- Human Bronchial Epithelial Cell Lines to Model Respiratory Lung Diseases

Related Product Categories
Classical Media & Buffers
Choose from a range of optimized cell culture media formulations, including DMEM, RPMI-1640, and serum-free options for diverse cellular needs.
MultiScreen® Filter Plates
Enhance your high-throughput screening capabilities using MultiScreen® filtration plates, ideal for ELISpot, ligand binding, and more.
Millipore® Sterile Filters
Seamless Sterile Filtration: Choose us for reliable Stericap® & Steripak® devices, ensuring sterile, quality solutions from mL to 20 L.
Stericup® Vacuum Filters
Next-gen Stericup® Quick Release: upgraded design enhances usability, streamlines filtration, safeguards cell cultures with Millipore® membranes.
Cell Counting Equipment
Ensure precise cell counts: handheld coulter counter delivers precise, rapid results. Opt for disposable hemocytometers in infectious environments.
CellASIC® ONIX2 Microfluidics
The CellASIC® ONIX2 Microfluidic System is a powerful, automated platform that allows precise manipulation of the cell culture environment for live-cell imaging and analysis using mammalian, yeast, and bacterial cells.
Mammalian Cell Lines
Cell lines from human and other mammalian species are essential for modeling disease and biological systems, and are critical tools for protein, antibody, virus, and vaccine production. We offer authenticated, contaminant-free cell lines, many in partnership with ECACC.
Primary Cells
Primary cells are derived directly from living tissue and closely replicate the physiology of biological systems. Our collection includes blood, patient tumor xenografts, and hepatocytes essential for drug screening and ADME/Tox studies.
Related Applications
Cell Growth & Maintenance
A synopsis of the conditions, media, nutrients, cultureware, and environments required for successful propagation of mammalian and yeast cells. Nutrient reagents, sterile technique and filtration, and requirements for 2D, 3D, and suspension cultures are described.
Mammalian Cell Culture
Applications, principles, and methods for in vitro mammalian cell culture including links to articles, protocols, and videos for proper aseptic technique, passaging and subculturing, and culture media considerations.
Primary Cell Culture
Primary cells isolated directly from human and animal sources maintain physiological relevance of the tissues from which they are derived, and are used for ADME/Tox screening. Find applications and methods for culturing mammalian primary cells in vitro.
3D Cell Culture
Applications, principles, and techniques for three-dimensional (3D) cell culture. Includes techniques and descriptions of scaffold and scaffold-free methods matrices for the culture of complex in vitro models including organoids and spheroids.
Immunohistochemistry
Immunohistochemistry (IHC) is a common immunostaining technique that is used by researchers to identify specific antigens of interest in healthy and diseased tissues.
Electron Microscopy
Electron microscopy utilizes a scanning or transmission system to pass an electron beam through a specimen to obtain ultrahigh resolution images with detailed structural information at the surface or atomic level.

Highlights

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Caco 2 Cells 21 Day Culture
Caco 2 Cells 21 Day Culture
Illustrating the Difference Between Plastic and 0.4 micron PC Membrane
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