CWDS-IF594
ColorWheel® Antibody-Ready iFluor 594 Dye
for use with ColorWheel® Antibody (Required, Sold Separately)
Synonym(s):
iFluor 594
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About This Item
UNSPSC Code:
12352203
Recommended Products
product line
ColorWheel®
Quality Level
form
lyophilized
purified by
ion-exchange chromatography
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
flow cytometry: suitable
fluorescence
λex 588 nm; λem 604 nm
compatibility
for use with ColorWheel® Antibody (Required, Sold Separately)
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
General description
ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
iFluor 594 is optimized for labeling antibodies and proteins. It is a pH-independent dye that works over a wide range of pH (3 to 11). It displays an excitation max at 588 nm and an emission max at 604 nm. Its spectral properties are similar to Texas Red® and ALEXA FLUOR™ 594. However, compared to Texas Red®, it is easier to conjugate with R-Phycoerythrin (RPE) with higher conjugation yield and the resulting RPE-iFluor 594 tandem dye offers better FRET efficiency. It exhibits decent stability shows good reactivity and selectivity with amino groups.
iFluor 594 is optimized for labeling antibodies and proteins. It is a pH-independent dye that works over a wide range of pH (3 to 11). It displays an excitation max at 588 nm and an emission max at 604 nm. Its spectral properties are similar to Texas Red® and ALEXA FLUOR™ 594. However, compared to Texas Red®, it is easier to conjugate with R-Phycoerythrin (RPE) with higher conjugation yield and the resulting RPE-iFluor 594 tandem dye offers better FRET efficiency. It exhibits decent stability shows good reactivity and selectivity with amino groups.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
Specificity
ColorWheel Antibody-Ready iFluor 594 Dye is designed for use with ColorWheel Dye-Ready antibodies in Flow cytometry.
Application
Flow Cytometry: Excitation: 588 nm, Emission: 604 nm.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Compatibility
Requires ColorWheel® Antibody (Sold Separately)
Physical form
CWDS-IF594-25UL Set Components
CWDS-IF594-25UL - ColorWheel® Antibody-Ready iFluor 594 Dye Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
CWD-FINISH-25UL - ColorWheel® Finishing Buffer Lyophilized from PBS. Contains no biocide or preservatives, such as azide.
CWDS-IF594-25UL - ColorWheel® Antibody-Ready iFluor 594 Dye Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
CWD-FINISH-25UL - ColorWheel® Finishing Buffer Lyophilized from PBS. Contains no biocide or preservatives, such as azide.
Reconstitution
Reconstitute each vial by adding 25 μL PBS.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany
Texas Red is a registered trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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