Recommended Products
product name
L6.G8.C5, 92121114
biological source
rat muscle
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat skeletal muscle myoblast
Cell Line Description
A subclone of L6, a rat thigh muscle cell line. On reaching confluence the cells will fuse to form myotubes and twitching movement will be seen. Once cultures have fused they cannot be recloned.
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split semi-confluent cultures to a seeding density of 1.5-2x1,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells fuse on reaching confluence, producing myotubes. To prevent fusion maintain the cultures sub-confluent and reclone every 6-8 weeks.
Other Notes
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Certificates of Analysis (COA)
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