MMYOMAG-74K
MILLIPLEX® Mouse Myokine Magnetic Bead Panel
Inflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enable the simultaneous analysis of multiple myokine and cytokine biomarkers in mouse serum and plasma samples.
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47
Recommended Products
Quality Level
species reactivity
mouse
manufacturer/tradename
Milliplex®
assay range
accuracy: 3-40,000 pg/mL
(Osteocrin)
standard curve range: 0.6-9,000 pg/mL
(BDNF)
standard curve range: 128-2,000,000 pg/mL
(Osteonectin (SPARC))
standard curve range: 16-250,000 pg/mL
(Oncostatin M)
standard curve range: 22-350,000 pg/mL
(EPO)
standard curve range: 256-4,000,000 pg/mL
(Myostatin)
standard curve range: 26-400,000 pg/mL
(26Irisin)
standard curve range: 3-40,000 pg/mL
(LIF)
standard curve range: 3-40,000 pg/mL
(Osteocrin/Musclin (OSTN))
standard curve range: 48-750,000 pg/mL
(IL-15)
standard curve range: 5-75,000 pg/mL
(FGF21)
standard curve range: 9-140,000 pg/mL
(IL-6)
inter-assay cv: <15%
intra-assay cv: <10%
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
shipped in
ambient
General description
Skeletal muscle is actively involved in the synthesis and secretion of many proteins collectively termed “myokines.” Muscle contraction during physical activity is an important activator for the release of myokines, which act in an autocrine and/or paracrine manner to regulate skeletal muscle growth. Myokines also act as endocrine hormones which mediate inter-organ crosstalk. Myokine theory provides molecular mechanistic explanations for exercise-induced metabolic changes in liver and adipose tissue as well as for profound changes in immune and neuron systems. Various genetically engineered mouse strains and disease models are now serving as exceptional research tools for human myokine study.
MILLIPLEX® Mouse Myokine Panel is a 12-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, and tissue culture samples: Brain-derived Neurotrophic Factor (BDNF), Erythropoeitin (EPO)*, Fibroblast Growth Factor 21 (FGF21), Follistatin-Like Protein 1 (FSTL-1), IL-6, IL-15, Irisin, Leukemia Inhibitory Factor (LIF), Myostatin (MSTN / GDF8), Oncostatin M (OSM), Osteocrin/Musclin (OSTN), Osteonectin (SPARC).
*Only available for cells culture samples.
This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Metabolism
MILLIPLEX® Mouse Myokine Panel is a 12-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, and tissue culture samples: Brain-derived Neurotrophic Factor (BDNF), Erythropoeitin (EPO)*, Fibroblast Growth Factor 21 (FGF21), Follistatin-Like Protein 1 (FSTL-1), IL-6, IL-15, Irisin, Leukemia Inhibitory Factor (LIF), Myostatin (MSTN / GDF8), Oncostatin M (OSM), Osteocrin/Musclin (OSTN), Osteonectin (SPARC).
*Only available for cells culture samples.
This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Metabolism
Application
- Analytes: Brain Derived Neurotrophic Factor (BDNF), Erythropoietin (EPO), Fibroblast Growth Factor 21 (FGF-21), Follistatin-like Protein 1 (FSTL1), IL-6, IL-15, Irisin, Leukemia Inhibitory Factor (LIF), Myostatin (MSTN/GDF8), Oncostatin M (OSM), Osteocrin (OSTN/Musclin), Osteonectin (SPARC)
- NOTES: BDNF - Substantial amounts of BDNF are stored in circulating platelets and subsequently released upon platelet activation. Therefore, platelet-poor plasma is critical to ensure accurate measurement of circulating levels of BDNF. OSM - While OSM is barely detectable in the serum/plasma of healthy subjects, a slight increase can be observed in certain disease states. Higher measurements in cell culture samples have also been reported. Erythropoeitin (EPO) is for tissue culture samples only and cannot be combined with other analytes in this panel when measuring serum or plasma.
- Recommended Sample Type: Mouse serum, plasma, or cell/tissue culture supernatants or lysates
- Recommended Sample Dilution: 25 μL per well of 1:2 diluted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
- Assay Run Time: For greater sensitivity an overnight incubation is recommended, (16-18 hours) at 2-8°C. Alternatively, incubate for 2 hours at room temperature (20-25°C).
- Research Category: Metabolism
- Research Subcategory: Obesity, Metabolic Disorders, Inflammation
Features and Benefits
Design your multiplex kit by choosing available analytes within this panel.
Other Notes
Standard curve range: See protocol
Legal Information
6538 is a trademark of American Type Culture Collection
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Target Organs
Respiratory Tract
WGK
WGK 3
Certificates of Analysis (COA)
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Sofia Garcia et al.
Aging cell, 17(2) (2018-02-11)
PGC-1α is a transcriptional co-activator known as the master regulator of mitochondrial biogenesis. Its control of metabolism has been suggested to exert critical influence in the aging process. We have aged mice overexpressing PGC-1α in skeletal muscle to determine whether
Susana Peralta et al.
Journal of cell science, 131(13) (2018-06-15)
Mutations in the mitochondrial inner membrane ATPase ATAD3A result in neurological syndromes in humans. In mice, the ubiquitous disruption of Atad3 (also known as Atad3a) was embryonic lethal, but a skeletal muscle-specific conditional knockout (KO) was viable. At birth, ATAD3
Lars Karlsson et al.
Molecular neurobiology, 58(4), 1465-1481 (2020-11-18)
Physical exercise can improve age-dependent decline in cognition, which in rodent is partly mediated by restoration of an age-dependent decline in neurogenesis. Exercise-inducible myokines in the circulation present a link in muscle-brain crosstalk. The transcription factor PGC-1α regulates the release
Edward O List et al.
Endocrinology, 160(1), 68-80 (2018-11-22)
Global GH receptor-null or knockout (GHRKO) mice have been extensively studied owing to their unique phenotype (dwarf and obese but remarkably insulin sensitive and long-lived). To better understand the influence of adipose tissue (AT) on the GHRKO phenotype, we previously
Adaptive thermogenesis enhances the life-threatening response to heat in mice with an Ryr1 mutation.
Hui J Wang et al.
Nature communications, 11(1), 5099-5099 (2020-10-11)
Mutations in the skeletal muscle Ca2+ release channel, the type 1 ryanodine receptor (RYR1), cause malignant hyperthermia susceptibility (MHS) and a life-threatening sensitivity to heat, which is most severe in children. Mice with an MHS-associated mutation in Ryr1 (Y524S, YS)
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