MABS2250
Anti-phospho-VCP (Ser784) Antibody, clone 3E4
Synonym(s):
Transitional endoplasmic reticulum ATPase;EC:3.6.4.6;TER ATPase;15S Mg(2+)-ATPase p97 subunit;Valosin-containing protein
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About This Item
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
3E4, monoclonal
mol wt
calculated mol wt 89.32 kDa
observed mol wt ~95 kDa
purified by
using protein G
species reactivity
human
species reactivity (predicted by homology)
bovine, porcine, canine, rat, monkey, mouse
Related Categories
1 of 4
This Item | A2859 | M2444 | SAB4200272 |
---|---|---|---|
conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
biological source mouse | biological source mouse | biological source mouse | biological source mouse |
Gene Information human ... ASF1A(25842) | Gene Information human ... ATG5(9474) | Gene Information human ... MDC1(9656) | Gene Information human ... LMNA(4000) |
antibody form purified from hybridoma cell culture | antibody form purified immunoglobulin | antibody form purified from hybridoma cell culture | antibody form purified from hybridoma cell culture |
species reactivity mouse, human | species reactivity human, mouse, rat | species reactivity monkey, human | species reactivity human |
General description
Specificity
Immunogen
Application
Evaluated by Western Blotting in lysates from HeLa cells treated with etoposide.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected VCP phosphorylated on serine 784 in lysates from HeLa cells treated with etoposide (10 μM; 24 h), but not in untreated cells.
Tested Applications
Western Blotting Analysis: A representative lot detected phospho-VCP (Ser784) in Western Blotting applications (Zhu, C., et al. (2020). Cell Rep. 31(10):107745).
Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho-VCP (Ser784) in Immunoprecipitation applications (Zhu, C., et al. (2020). Cell Rep. 31(10):107745).
Immunocytochemistry Analysis: A representative lot detected phospho-VCP (Ser784) in Immunocytochemistry applications (Zhu, C., et al. (2020). Cell Rep. 31(10):107745).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Physical form
Storage and Stability
Other Notes
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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