MABC537
Anti-Vasohibin-1 Antibody, clone 4E12 (Azide Free)
clone 4E12, 1 mg/mL, from mouse
Synonym(s):
Vasohibin
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4E12, monoclonal
species reactivity
human
concentration
1 mg/mL
technique(s)
activity assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... VASH1(22846)
General description
Vasohibin-1 is an endothelium-derived negative feedback regulator of angiogenesis. Vasohibin-1 inhibited migration, proliferation, and network formation by ECs in culture, as well as angiogenesis in vivo, and Vasohibin-1 was selective for endothelial cells and its influence was eliminated by hypoxia or TNF-alpha interaction. Vasohibin is preferentially expressed in endothelial cells and does not appear to promote proliferation or tumor growth when over expressed. Vasohibin-1 expression is induced by VEGF expression and its effects are mediated through VEGF-R2 independent of VEGF-R1 and Vasohibin-1 may directly influence the expression of VEGF-R2. Because the inhibition effects of Vasohibin-1 are nullified by hypoxia and various cytokines it is thought that this is how tumors escape this built-in negative regulator of angiogenesis. Vasohibin-1 is highly expressed in fetal organs and the endothelial cells during development, and also expressed in the brain and placental and microvessels endothelial cells of atherosclerotic lesions in the adult.
Immunogen
KLH-conjugated linear peptide corresponding to Human Vasohibin-1.
Application
Functional Activity Assay: A representative lot of this antibody induced cellular senescence (Miyashita, H., et al. (2012) PLOS ONE. 7(10):e46459).
Western Blotting Analysis: A representative lot of this antibody detected Vasohibin-1 in HUVECs transfected with control siRNA, but not in HUVECs transfected with Vasohibin-1 siRNA (Miyashita, H., et al. (2012) PLOS ONE. 7(10):e46459).
Western Blotting Analysis: A representative lot of this antibody detected Vasohibin-1 (KIAA1036 protein referenced in publication) in VEGF-stimulated HUVECs and GM7373 transfected cell lysate (Watanabe, K,, et al. (2004) JCI. 114(7):898-907).
Immunohistochemistry Analysis: A representative lot of this antibody detected Vasohibin-1 (KIAA1036 protein referenced in publication) in human placenta tissue (Watanabe, K,, et al. (2004) JCI. 114(7):898-907).
Immunocytochemistry Analysis: A representative lot of this antibody detected Vasohibin-1 (KIAA1036 protein referenced in publication) in HUVEC cells (Watanabe, K,, et al. (2004) JCI. 114(7):898-907).
Western Blotting Analysis: A representative lot of this antibody detected Vasohibin-1 in HUVECs transfected with control siRNA, but not in HUVECs transfected with Vasohibin-1 siRNA (Miyashita, H., et al. (2012) PLOS ONE. 7(10):e46459).
Western Blotting Analysis: A representative lot of this antibody detected Vasohibin-1 (KIAA1036 protein referenced in publication) in VEGF-stimulated HUVECs and GM7373 transfected cell lysate (Watanabe, K,, et al. (2004) JCI. 114(7):898-907).
Immunohistochemistry Analysis: A representative lot of this antibody detected Vasohibin-1 (KIAA1036 protein referenced in publication) in human placenta tissue (Watanabe, K,, et al. (2004) JCI. 114(7):898-907).
Immunocytochemistry Analysis: A representative lot of this antibody detected Vasohibin-1 (KIAA1036 protein referenced in publication) in HUVEC cells (Watanabe, K,, et al. (2004) JCI. 114(7):898-907).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Tumor Markers
Tumor Markers
This Anti-Vasohibin-1 Antibody, clone 4E12 (Azide Free) is validated for use in Western Blotting and Immunohistochemistry and Immunocytochemistry and Activity Assay for the detection of Vasohibin-1.
Quality
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Vasohibin-1 in 10 µg of HEK293 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Vasohibin-1 in 10 µg of HEK293 cell lysate.
Target description
~ 41 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing PBS without preservatives.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Kazuhide Watanabe et al.
The Journal of clinical investigation, 114(7), 898-907 (2004-10-07)
Negative feedback is a crucial physiological regulatory mechanism, but no such regulator of angiogenesis has been established. Here we report a novel angiogenesis inhibitor that is induced in endothelial cells (ECs) by angiogenic factors and inhibits angiogenesis in an autocrine
Hiroki Miyashita et al.
PloS one, 7(10), e46459-e46459 (2012-10-12)
Vasohibin-1 (VASH1) is isolated as an endothelial cell (EC)-produced angiogenesis inhibitor. We questioned whether VASH1 plays any role besides angiogenesis inhibition, knocked-down or overexpressed VASH1 in ECs, and examined the changes of EC property. Knock-down of VASH1 induced premature senescence
Siem van der Laan et al.
Cell reports, 29(12), 4159-4171 (2019-12-19)
The two related members of the vasohibin family, VASH1 and VASH2, encode human tubulin detyrosinases. Here we demonstrate that, in contrast to VASH1, which requires binding of small vasohibin binding protein (SVBP), VASH2 has autonomous tubulin detyrosinating activity. Moreover, we
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