475981

Myosin Light Chain Kinase Inhibitor Peptide 18

The Myosin Light Chain Kinase Inhibitor Peptide 18 controls the biological activity of Myosin Light Chain Kinase. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

• Synonym(s): Myosin Light Chain Kinase Inhibitor Peptide 18, H-RKKYKYRRK-NH₂, MLCK Inhibitor Peptide 18, MLCK Inhibitor IV, MLCK Inhibitor Peptide 18, H-RKKYKYRRK-NH₂, MLCK Inhibitor IV
• Empirical Formula (Hill Notation): C₆₀H₁₀₅N₂₃O₁₁
• Molecular Weight: 1324.62
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥97% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• color: white
• solubility: water: 1 mg/mL; DMSO: 5 mg/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

A highly basic, cell-permable, nonapeptide that acts as a selective myosin light chain kinase (MLCK) inhibitor (IC₅₀ = 50 nM). Does not block calmodulin (CaM) or inhibit the kinase activities of Ca²⁺/calmodulin kinase II (CaMKII) or protein kinase A (PKA). Inhibition is competitive with respect to substrate (K_i = 52 nM). Displays mixed mode inhibition with respect to ATP.

A highly basic, cell-permeable, nonapeptide that acts as a selective Myosin Light Chain Kinase (MLCK) inhibitor (IC₅₀ = 50 nM). The inhibition is competitive with respect to the peptide substrate (K_i = 52 nM) and displays a mixed mode of inhibition with respect to ATP. Does not interfere with kinase activation by calmodulin (CaM), nor does it inhibit the activities of CaMKII and PKA.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Mlck

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 50 nM against MLCK

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

H-Arg-Lys-Lys-Tyr-Lys-Tyr-Arg-Arg-Lys-NH₂

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Zolotarevsky, Y., et al. 2002. Gastroenterology123, 163.
Lukas, T.J., et al. 1999. J. Med. Chem.42, 910.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable