(ELISA) Enzyme-Linked Immunosorbent Assay

Typically performed in multi-well microtiter plates, ELISAs are a molecular biology assay commonly used for the detection and quantification of diverse molecules, including peptides, proteins, and antibodies. Such assays can detect molecules of interest at the pg/mL level and are critical for both basic research and disease research application needs.

ELISA Assays: Antibody-Antigen Interactions

The fundamental molecular components of an ELISA typically include the use of antibody conjugated to an enzyme, an immobilized molecule(s) of interest, and a detection substrate. A critical aspect that determines the success and quality of data obtained from an ELISA is dependent on the affinity and specificity of antibody-antigen interactions. Antigen-antibody interactions are influenced by numerous factors, including pH, temperature, and ionic strength.