SRP6262

VEGFR1/Flt-1 human

recombinant, expressed in HEK 293 cells, ≥98% (SDS-PAGE)

• Synonym(e): FLT, VEGFR1
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in HEK 293 cells
• Markierung: 6-His tagged (N-terminus)
• Assay: ≥98% (SDS-PAGE)
• Form: lyophilized powder
• Mol-Gew.: calculated mol wt 50.6 kDa; observed mol wt 55-60 kDa (DTT-reduced. Protein migrates due to glycosylation. Asp 34 is the predicted N-terminus.)
• Verpackung: pkg of 10 μg
• Verunreinigungen: <1 EU/μg endotoxin (LAL test)
• UniProt-Hinterlegungsnummer: P17948
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... VEGFR1(2321)

Beschreibung

Allgemeine Beschreibung

Vascular endothelial growth factor receptor 1 (VEGFR1) also known as Fms-like tyrosine kinase 1 (FLT-1), Tyrosine-protein kinase receptor FLT, is a single-pass type I membrane protein and secreted protein which belongs to the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. VEGFR1 is detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues and specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. VEGFR1 acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. VEGFR1 may play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. VEGFR1 can promote endothelial cell proliferation, survival and angiogenesis in adulthood.

Physikalische Form

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable