SRP0723

YSK4 (MAP3K19), Active

PRECISIO^® Kinase, recombinant

• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Rekombinant: expressed in baculovirus infected Sf9 cells
• Assay: 70-95% (SDS-PAGE)
• Form: frozen liquid
• Spezifische Aktivität: 30-45 nmol/min·mg (radiometric)
• Mol-Gew.: ~83 kDa
• Konzentration: 0.05 μg/μL
• Hinterlegungsnummer: NM_025052
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... YSK4(80122)

Beschreibung

Anwendung

Useful for kinase assay and Western blot.

Biochem./physiol. Wirkung

Recombinant human YSK4 (MAP3K19) (941-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. YSK4 (MAP3K19, RCK) belongs to STE Ser/Thr protein kinase family and STE20 subfamily. The YSK4 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, and rat ⁽¹⁾. The important paralog is MAP3K1 gene. The RCK ligand-binding domains form an octameric gating ring ⁽²⁾ in the large-conductance Ca²⁺-activated K⁺ channels ⁽³⁾. The YSK4 kinase function has not been made clearly yet.

Rekonstituierung

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Lagerung und Haltbarkeit

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Rechtliche Hinweise

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable