N8010

4-Guanidino-benzoesäure-4-nitrophenylester -hydrochlorid

protease substrate, ≥95.0% (TLC), powder

• Synonym(e): 4-Nitrophenyl-4-Guanidinobenzoat -hydrochlorid, pNPGB
• Empirische Formel (Hill-System): C₁₄H₁₂N₄O₄ · HCl
• CAS-Nummer: 19135-17-2
• Molekulargewicht: 336.73
• Beilstein: 8024554
• EG-Nummer: 242-831-3
• MDL-Nummer: MFCD00037010
• UNSPSC-Code: 12352204
• PubChem Substanz-ID: 24897847
• NACRES: NA.32

Eigenschaften

• product name: 4-Guanidino-benzoesäure-4-nitrophenylester -hydrochlorid, protease inhibitor and substrate
• Qualitätsniveau: 200
• Assay: ≥95.0% (TLC)
• Form: powder
• Löslichkeit: formic acid: soluble 49.00-51.00 mg/mL
• Lagertemp.: −20°C
• SMILES String: Cl[H].NC(=N)Nc1ccc(cc1)C(=O)Oc2ccc(cc2)[N+]([O-])=O
• InChI: 1S/C14H12N4O4.ClH/c15-14(16)17-10-3-1-9(2-4-10)13(19)22-12-7-5-11(6-8-12)18(20)21;/h1-8H,(H4,15,16,17);1H
• InChIKey: PKSBDZOBYIKNGY-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

4-Nitrophenyl 4-guanidinobenzoate hydrochloride (pNPGB) is a trypsin substrate. It is more water-soluble than 4-methylumbelliferyl 4-guanidinobenzoate and is preferred in spectrophotometric assay.

Anwendung

4-Nitrophenyl 4-guanidinobenzoate hydrochloride has been used:

• as a substrate for trypsin for active site titration experiments
• for pre-treating of mosquito eggs in the interplasmid transposition assay
• as a component of isotonic buffer to moisten filter paper for mosquito embryo collection

Biochem./physiol. Wirkung

4-Nitrophenyl 4-guanidinobenzoate hydrochloride (pNPGB) inhibits phenoloxidase enzyme and delays embryo chorionic membrane hardening.

Sicherheitshinweise

• Piktogramme: GHS05
• Signalwort: Danger
• H-Sätze: H318
• P-Sätze: P280 - P305 + P351 + P338
• Gefahreneinstufungen: Eye Dam. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Gloves