GE17-5280-01

nProtein A Sepharose^™ 4 Fast Flow

Cytiva 17-5280-01, pack of 5 mL

• UNSPSC-Code: 41106500
• NACRES: NA.56

Eigenschaften

• ligand: native protein A (S. aureus)
• Haltbarkeit: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
• Verpackung: pack of 5 mL
• Hersteller/Markenname: Cytiva 17-5280-01
• Lagerbedingungen: (20% Ehtanol)
• Matrix: 4% cross-linked agarose
• Durchschnittlicher Durchmesser: 90 μm (d50v)
• cleaning: 2-10(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
• working range: 3-9
• Kapazität: >30 mg binding capacity(human IgG/ml)
• Eignung: suitable for bioprocess medium
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

nProtein A Sepharose^™ 4 Fast Flow is native protein A coupled to Sepharose^™ 4 Fast Flow for the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.

nProtein A Sepharose^™ 4 Fast Flow is native protein A coupled to the well established Sepharose^™ 4 Fast Flow base matrix. The native protein A ligand is produced by fermenting a selected strain of Staphylococcus aureus. The purified protein is coupled to the cross-linked 4% agarose base matrix by the cyanogen bromide technique, giving a highly stable medium with minimal non-specific adsorption. nProtein A Sepharose^™ 4 Fast Flow is manufactured without using animal-derived components.

nProtein A Sepharose^™ 4 Fast Flow has nearly twice the total IgG binding capacity of Protein A Sepharose^™ CL-4B, and is suitable for recovery and purification of monoclonal antibodies from cell culture at both laboratory and process scale. nProtein A Sepharose^™ 4 Fast Flow was developed and tested in co-operation with leading manufacturers of purified monoclonal antibody products, and is used in routine commercial production.

As member of the BioProcess media range, nProtein A Sepharose^™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Leistungsmerkmale und Vorteile

• Replaces Protein A Sepharose^™ 4 Fast Flow, the first Cytiva Protein A medium for large-scale purification of antibodies.
• Used in routine commercial production of monoclonal antibodies
• Free from animal-derived components.

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

Sepharose is a trademark of Cytiva

Sicherheitshinweise

• Piktogramme: GHS02
• Signalwort: Warning
• H-Sätze: H226
• P-Sätze: P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501
• Lagerklassenschlüssel: 3 - Flammable liquids