HT 1197

87032403, human bladder, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: HT 1197, 87032403
• Biologische Quelle: human bladder
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 46
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian bladder carcinoma

Beschreibung der Zelllinie

Derived from a grade 4 transitional cell carcinoma of the bladder of a 44 year old Caucasian male who had not received chemo or radiation therapy. The cells grow in soft agar, produce fibrinolytic activity and are tumorigenic in mice and hamsters.

Anwendung

HT 1197 has been used to study the transcription activity of human endogenous retroviruses (HERV).

DNA-Profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,12
D16S539: 12,13
D5S818: 12
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 16,18

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2x10,000 to 5x100,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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