Mycl-9E10

Reactivity: Anti-c-Myc gene product, human, 85102202

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Myelom: Sp2/0
• Reaktivität: Anti-c-Myc gene product, human
• Methode(n): cell culture | mammalian: suitable
• Isotyp: IgG1, kappa
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse Sp2/0 x Mouse BALB/c spleen

Beschreibung der Zelllinie

The antibody recognises human p62c-myc gene product and is specific for C-terminal peptide G. The antibody failed to immunoprecipitate p62 c-myc, but recognised the imunoblotted protein. Compared to other hybridomas specific to this antigen (Mycl-8F9, Mycl-3C7, CT9-B7, CT14-B2) Mycl-9E10 is the most sensitive probe for immunoblots. Conformation of antigen is critical for antibody recognition. No reported cross-reaction, with chicken p110gag-myc protein or mouse c-myc protein. This cell line is available as a growing culture only to customers in Europe.

Immunogen

A synthetic peptide, corresponding to residues 408-432(C-terminus of Peptide G) of human p62c-myc sequence, conjugated to KLH

Nährmedium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subkultur-Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cells are slow to grow. This cell line is available as a growing culture only to customers in Europe.

Sonstige Hinweise

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