BHK21C13-3P

84100501, hamster kidney, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• product name: BHK21C13-3P, 84100501
• Biologische Quelle: hamster kidney
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 44, pseudodiploid
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Hamster Syrian kidney

Beschreibung der Zelllinie

BHK21C13-3P is a subclone of the parent line BHK-21 (Clone-13) (Sigma Catalogue number. 85011433). The cells grow as monolayers and have the same virus susceptibility as the parent line.

Anwendung

Virus replication studies

Nährmedium

GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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