T7

7021402, mouse lung, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: T7, 07021402
• Biologische Quelle: mouse lung
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse Type-II lung epithelial cell

Beschreibung der Zelllinie

Fully differentiated Type-II alveolar epithelial cell line derived from transgenic H-2Kb-tsA58 mice capable of being passaged as an immortalised cloned cell line in culture. Cells will proliferate at 33 - 35°C in the presence of gamma-interferon, at 39°C in the absence of gamma-interferon cells will terminally differentiate. Supplied as frozen ampoules only.

Nährmedium

DMEM + 2mM Glutamine + 4% Foetal Bovine Serum (FBS) + 8 additional supplements - please see ′Additional Literature Report′ field below for full details. ECACC can supply an information sheet on the preparation of the supplements on request - contact ECAC

Subkultur-Routine

Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA;10% CO₂; 33°C. Doubling time 48h

Sonstige Hinweise

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