KARPAS-929

06072606, human bone marrow, round to polygonal cells growing in clusters in suspension

• Synonym(e): KARPAS 929, KARPAS, 929, KARPAS929
• UNSPSC-Code: 41106514

Eigenschaften

• product name: KARPAS-929, from human bone marrow, 06072606
• Biologische Quelle: human bone marrow
• Wachstumsmodus: Suspension
• Karyotyp: Hyperdiploid with complex aberrant chromosomes
• Morphologie: Round to polygonal cells growing in clusters in suspension
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice

Beschreibung

Ursprung der Zelllinie

Human multiple myeloma

Beschreibung der Zelllinie

Established in 1984 from the bone marrow of a 67-year old woman diagnosed with myeloma who survived for one year after diagnosis.

DNA-Profil

Not specified

Nährmedium

RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS)

Subkultur-Routine

Doubling time approximately 30 hours. Cells grow singly in suspension. Maintain cultures between 5-20 x 10⁵ cells/ml; 5% CO₂; 37 °C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 5 x 10⁵ cells/ml. At subculture perform a cell count and maintain cultures between 5-20 x 10⁵ cells/ml, this tends to equate to a 1:3 split every 2-3 days.

Sonstige Hinweise

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Haftungsausschluss

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.