PF126

Bcl-2, C-Terminal Truncated, His•Tag^®, Human, Recombinant, E. coli

Recombinant, human Bcl-2 without the 17 amino acid mitochondrial targeting sequence at the C-terminus, fused to a His•Tag sequence, and expressed in E. coli.

• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 100
• Rekombinant: expressed in E. coli
• Assay: ≥95% (SDS-PAGE)
• Form: liquid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Versandbedingung: wet ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Recombinant, human Bcl-2 without the 17 amino acid mitochondrial targeting sequence at the C-terminus, fused to a His•Tag sequence, and expressed in E. coli. The truncated protein retains the ability to neutralize pro-apoptotic Bcl-2 family members.

Recombinant, human Bcl-2 without the 17 amino acid mitochondrial targeting sequence at the C-terminus, fused to a His-Tag sequence and expressed in E. coli. The truncated protein retains the ability to neutralize pro-apoptotic Bcl-2 family members.

Anwendung

Neutralization (see biological activity)

Biochem./physiol. Wirkung

EC₅₀ = 100-300 nM based on its ability to inhibit 54 nM caspase-8-cleaved human Bid-mediated release of cytochrome c from isolated mouse liver mitochondria

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

In 100 mM KCl, 10 mM HEPES, 20% glycerol, pH 8.0.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C).

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable