MABE982

Anti-RBP-Jk, clone 1F1 Antibody

clone 1F1, from rat

• Synonym(e): Recombining binding protein suppressor of hairless, CBF-1, J kappa-recombination signal-binding protein, RBP-J kappa, RBP-J, RBP-JK, Renal carcinoma antigen NY-REN-30
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rat
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 1F1, monoclonal
• Speziesreaktivität: Drosophila, human
• Methode(n): ChIP: suitable; electrophoretic mobility shift assay: suitable; immunoprecipitation (IP): suitable
• Isotyp: IgG2aκ
• NCBI-Hinterlegungsnummer: NP_005340
• UniProt-Hinterlegungsnummer: Q06330
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... RBPJ(3516)

Beschreibung

Allgemeine Beschreibung

RBP-jk, Recombinant signal binding protein for immunoglobin kappa J region, is a human homolog for the Drosophila gene, suppressor of hairless. RBP-jk is located in the nucleus and is a 60 kDa monomeric protein that is used as a promoter region in notch signaling. Notch signaling is important in cell to cell communication which allows "a differentiating cell to suppress the same differentiation fate in a neighboring cell (the receiving cell). This ensures that the correct number of cells are distributed to distinct cell fates." When RBP-jk is not associated with Notch proteins, it acts as a transcriptional repressor. A disease associated with RBP-jk is the Epstein Barr virus.

Immunogen

Recombinant protein corresponding to human RBP-Jk.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP-Jk using Lymphoblastoid cell line.

Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP-Jk using DG75 cells.

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP-Jk using Hippocampal NSC′s (Ehm, O., et al. (2010). Journal of Neuroscience. 30(41):13794-18707).

Electrophoretic Mobility Shift Assay: A representative lot detected RBP-Jk using Hippocampal lysate (Ehm, O., et al. (2010). Journal of Neuroscience. 30(41):13794-18707).

Research Sub Category
Nuclear Receptors

This Anti-RBP-Jk, clone 1F1 Antibody is validated for use in Immunoprecipitation and Chromatin Immunoprecipitation (ChIP) and Electrophoretic Mobility Shift Assay for the detection of RBP-Jk.

Qualität

Evaluated by Immunoprecipitation in HeLa cell lysate.

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated RBP-Jk in 0.5 mg of HeLa cell lysate.

Zielbeschreibung

~55 kDa observed

Physikalische Form

Protein G Purified

Format: Purified

Purified rat IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable