Dual-Labeled Probes are the most common probe type for qPCR and are often referred to as hydrolysis probes.
Choose Dual-Labeled Probes for:
- Microarray validation
- Multiply target genes / few samples
- Pathogen detection
- Multiplexing
- Viral load quantification
- Gene expression analysis
- Gene copy determination
Perfect for validating MISSION® siRNA and shRNA knockdowns.
Benefits of Using Dual-Labeled Probes include:
- Design simplicity for sequence specificity
- Extensive availability of reporter/quencher combinations
- Increased sensitivity
Add Locked Nucleic Acid to Your Probe for:
- Increased thermal stability and hybridization specificity
- Greater accuracy in SNP detection, allele discrimination, and in vitro quantification or detection
- Easier and more sensitive probe designs for problematic target sequences
How Dual-Labeled Probes Work
A Dual-Labeled Probe is a single-stranded oligonucleotide labeled with two different dyes. A reporter dye is located at the 5’ end and a quencher molecule located at the 3’ end. The quencher molecule inhibits the natural fluorescence emission of the reporter by fluorescence resonance energy transfer (FRET). The illustration below depicts the mechanism.
![dual-labeled-probes Dual-Labeled Probes Work](/deepweb/assets/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/qpcr/dual-labeled-probes/dual-labeled-probes.jpg)
The primer is elongated by the polymerase and the probe binds to the specific DNA template. Hydrolysis releases the reporter from the probe/target hybrid, causing an increase in fluorescence. The measured fluorescence signal is directly proportional to the amount of target DNA.
Product Features include:
- Amounts: 1, 3, 5, and 10 OD
- Purification: HPLC
- Sequence Lengths: 15 - 40 bases
- Quality Control: 100% mass spectrometry
- Format: Supplied dry in amber tubes
- Custom formats available (normalization, special plates, etc.)
Our probes are provided in a format to simplify your experimental planning.
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1JOE/TET alternative
2VIC® alternative
3Cyanine 3 alternative
4TAMRA™ alternative
5Cyanine 5 alternative
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