Custom DNA and RNA Oligos & qPCR Probes Product Resources
For over 35 years we have been providing custom and predesigned oligos and qPCR probes for PCR, cloning, DNA sequencing, and gene expression.
We offer tools for oligo and qPCR probe design including OligoArchitect™ Primer and Probe Design Solutions and OligoEvaluator™: Oligonucleotide Sequence Calculator that provides primer analysis values for PCR and helps determine volumes for resuspension and dilution of your DNA oligos to desired final concentrations.
Featured Articles and Protocols
- Coronavirus qPCR Primer and probe sets for the detection of SARS-CoV-2 (Corona Virus), with additional real-time RT-PCR, RT-qPCR and supporting reagents available.
- We offer OligoEvaluator™ as an easy-to-use Oligo Analyzer tool for online oligonucleotide sequence calculator that provides primer dimer analysis values for PCR and calculation of Tm.
- Learn about the steps in phosphoramidite solid-phase method of Oligonucleotide Synthesis.
- Annealing is the process of heating and cooling two single-stranded oligonucleotides with complementary sequences.
Technical Literature
- Learn about the steps in phosphoramidite solid-phase method of Oligonucleotide Synthesis.
- Discover the best purification option for your oligo, based on the intended application.
- DNA can be easily quantitated in a UV spectrometer due to its highly conjugated nature.
- Phosphorothioate is a widely used backbone variant effective in creating oligonucleotides for antisense knockdowns.
- The ultimate goal of most antisense research is to develop therapeutic agents for diseases such as diabetes, cancer, and HIV/ AIDS. Phosphorothioate oligonucleotides (commonly referred to as S-oligos) are often the molecules of choice because of their resistance to cellular nuclease degradation.
- View a summary of the applications using commonly ordered custom qPCR probes.
- Learn more about how digital PCR (dPCR) is used for absolute quantification and for analysis of minority sequences.
- Frequently asked questions about Locked Nucleic Acids (LNA)
- Two commonly used quenchers, TAMRA™ and DABYCL, limit the ultimate sensitivity and flexibility of qPCR.
- Handing and stability techniques to reduce variation and ensure trouble-free experiments.
- Learn about methods for calculating oligonucleotide melting temperature (Tm).
Protocols, Procedures, Safety & Quality
- Protocol for conjugating NHS-ester modifications to an oligonucleotide with an amino label.
- Annealing is the process of heating and cooling two single-stranded oligonucleotides with complementary sequences.
- Follow this DDT reduction protocol to reduce disulfide bonds in thiol-modified oligonucleotides.
- Safety Data Sheets for Oligonucleotides.
- The foundation of Sigma's manufacturing process is a robust QMS, which drives compliance to ISO 9001:2008 certification.
- We are committed to product quality, manufacturing effectiveness and meeting customer expectations.
Videos
Product Citations & Our Publications
1.
Guo Y, Ye JY, Divin C, Huang B, Thomas TP, Baker, Jr. JR, Norris TB. 2010. Real-Time Biomolecular Binding Detection Using a Sensitive Photonic Crystal Biosensor. Anal. Chem.. 82(12):5211-5218. https://doi.org/10.1021/ac100576y
2.
Kudla G, Murray AW, Tollervey D, Plotkin JB. 2009. Coding-Sequence Determinants of Gene Expression in Escherichia coli. Science. 324(5924):255-258. https://doi.org/10.1126/science.1170160
3.
Lacroix-Lamandé S, Rochereau N, Mancassola R, Barrier M, Clauzon A, Laurent F. Neonate Intestinal Immune Response to CpG Oligodeoxynucleotide Stimulation. PLoS ONE. 4(12):e8291. https://doi.org/10.1371/journal.pone.0008291
4.
Omumi A, Beach DG, Baker M, Gabryelski W, Manderville RA. 2011. Postsynthetic Guanine Arylation of DNA by Suzuki?Miyaura Cross-Coupling. J. Am. Chem. Soc.. 133(1):42-50. https://doi.org/10.1021/ja106158b
5.
Pandey M, Syed S, Donmez I, Patel G, Ha T, Patel SS. 2009. Coordinating DNA replication by means of priming loop and differential synthesis rate. Nature. 462(7275):940-943. https://doi.org/10.1038/nature08611
6.
Wang Z, Elbaz J, Remacle F, Levine RD, Willner I. 2010. All-DNA finite-state automata with finite memory. Proceedings of the National Academy of Sciences. 107(51):21996-22001. https://doi.org/10.1073/pnas.1015858107
7.
Yang L, Kemadjou JR, Zinsmeister C, Bauer M, Legradi J, Müller F, Pankratz M, Jäkel J, Strähle U. 2007. Transcriptional profiling reveals barcode-like toxicogenomic responses in the zebrafish embryo. Genome Biol. 8(10):R227. https://doi.org/10.1186/gb-2007-8-10-r227
8.
Zykovich A, Korf I, Segal DJ. 2009. Bind-n-Seq: high-throughput analysis of in vitro protein?DNA interactions using massively parallel sequencing. 37(22):e151-e151. https://doi.org/10.1093/nar/gkp802
9.
Okabe K, Harada Y, Zhang J, Tadakuma H, Tani T, Funatsu T. 2011. Real time monitoring of endogenous cytoplasmic mRNA using linear antisense 2?-O-methyl RNA probes in living cells. 39(4):e20-e20. https://doi.org/10.1093/nar/gkq1196
1.
Josefsen MH, Löfström C, Sommer HM, Hoorfar J. 2009. Diagnostic PCR: Comparative sensitivity of four probe chemistries. Molecular and Cellular Probes. 23(3-4):201-203. https://doi.org/10.1016/j.mcp.2009.02.003
2.
Saito Y, Kitamura H, Hijikata A, Tomizawa-Murasawa M, Tanaka S, Takagi S, Uchida N, Suzuki N, Sone A, Najima Y, et al. 2010. Identification of Therapeutic Targets for Quiescent, Chemotherapy-Resistant Human Leukemia Stem Cells. Science Translational Medicine. 2(17):17ra9-17ra9. https://doi.org/10.1126/scitranslmed.3000349
1.
Schutkowski A, König B, Kluge H, Hirche F, Henze A, Schwerdtle T, Lorkowski S, Dawczynski C, Gabel A, Große I, et al. 2019. Metabolic footprint and intestinal microbial changes in response to dietary proteins in a pig model. The Journal of Nutritional Biochemistry. 67149-160. https://doi.org/10.1016/j.jnutbio.2019.02.004
2.
Schutkowski A, Max D, Bönn M, Brandsch C, Grundmann SM, Hirche F, Staege MS, Stangl GI. 2018. Vitamin D Does Not Play a Functional Role in Adipose Tissue Development in Rodent Models. Mol. Nutr. Food Res.. 62(4):1700726. https://doi.org/10.1002/mnfr.201700726
3.
Kühne H, Hause G, Grundmann SM, Schutkowski A, Brandsch C, Stangl GI. 2016. Vitamin D receptor knockout mice exhibit elongated intestinal microvilli and increased ezrin expression. Nutrition Research. 36(2):184-192. https://doi.org/10.1016/j.nutres.2015.10.005
4.
Schutkowski A, Wege N, Stangl GI, König B. Tissue-Specific Expression of Monocarboxylate Transporters during Fasting in Mice. PLoS ONE. 9(11):e112118. https://doi.org/10.1371/journal.pone.0112118
5.
Kühne H, Schutkowski A, Weinholz S, Cordes C, Schierhorn A, Schulz K, König B, Stangl GI. 2014. Vitamin D receptor regulates intestinal proteins involved in cell proliferation, migration and stress response. Lipids Health Dis. 13(1):51. https://doi.org/10.1186/1476-511x-13-51
1.
Hudnall SD, Chen T, Allison P, Tyring SK, Heath A. 2008. Herpesvirus prevalence and viral load in healthy blood donors by quantitative real-time polymerase chain reaction. Transfusion. 48(6):1180-1187. https://doi.org/10.1111/j.1537-2995.2008.01685.x
*qPCR primers and probes were used to detect virus in blood samples; a Custom Products scientist co-authored this paper.
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