HPLC Separation of an Oligonucleotide and Its Degradation Fragments on Proteomix^® SAX-NP5

column

Proteomix SAX-NP5, 15 cm x 4.6 mm I.D., 5 μm particles (Z777228)

mobile phase

(A) 25 mM Tris, 1 mM EDTA 10% acetonitrile (8.0 pH); (B) A + 1.0 M NaCl

gradient

0 to 75% B in 50 min

flow rate

0.5 mL/min

detector

sample

Oligonucleotide (MW 12196) and its degradation fragments