HPLC Analysis of FMOC-Threonine Enantiomers on Astec^® CHIROBIOTIC^® T

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

(A) 20 mM ammonium acetate, pH 4.1; (B) methanol; (60:40, A:B)

flow rate

0.8 mL/min

column temp.

25 °C

detector

UV, 260 nm

injection

1 μL

sample

1 mg/mL in methanol