HPLC Analysis of Beverage Additives in Diet Cola on Ascentis^® Express RP-Amide

column

Ascentis Express RP-Amide, 10 cm x 3.0 mm I.D., 2.7 μm particles (53918-U)

mobile phase

[A] 20 mM ammonium acetate, pH 4.7 with acetic acid; [B] acetonitrile; (92:8, A:B)

flow rate

0.6 mL/min

column temp.

40 °C

detector

UV, 214 nm

injection

1 μL

sample

500 - 1000 μg/mL in 95:5, buffer: acetonitrile