Saltar al contenido

Mixed alkyl aryl phosphonate esters as quenched fluorescent activity-based probes for serine proteases.

Mixed alkyl aryl phosphonate esters as quenched fluorescent activity-based probes for serine proteases.

Organic & biomolecular chemistry (2015-01-03)

Sevnur Serim, Philipp Baer, Steven H L Verhelst

RESUMEN

Activity-based probes (ABPs) are powerful tools for the analysis of active enzyme species in whole proteomes, cells or animals. Quenched fluorescent ABPs (qABPs) can be applied for real time imaging, allowing the visualization of dynamic enzyme activation by fluorescent microscopy. Unfortunately, qABPs are only available for a few enzymes. We here describe the design and synthesis of qABPs for serine proteases based on a phosphonate ester scaffold.

MATERIALES

Referencia del producto

Marca

Descripción del producto

Sigma-Aldrich

Ácido acético, glacial, ACS reagent, ≥99.7%

Sigma-Aldrich

Ácido acético, glacial, ReagentPlus^®, ≥99%

Sigma-Aldrich

Sulfato de magnesio, anhydrous, ReagentPlus^®, ≥99.5%

Sigma-Aldrich

2-Butanona, ACS reagent, ≥99.0%

Sigma-Aldrich

Sulfato de magnesio, anhydrous, reagent grade, ≥97%

Sigma-Aldrich

Ácido acético, glacial, ≥99.99% trace metals basis

Sigma-Aldrich

Ácido acético solution, suitable for HPLC

Sigma-Aldrich

Ácido acético, glacial, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, 99.8-100.5%

Sigma-Aldrich

Ácido acético, glacial, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%

Sigma-Aldrich

2-Butanona, suitable for HPLC, ≥99.7%

Sigma-Aldrich

(+)-Sodium L-ascorbate, crystalline, ≥98%

Sigma-Aldrich

Sulfato de magnesio, anhydrous, free-flowing, Redi-Dri^™, ReagentPlus^®, ≥99.5%

Sigma-Aldrich

(+)-Sodium L-ascorbate, BioXtra, ≥99.0% (NT)

Sigma-Aldrich

Dicarbonato de di-terc-butilo, ReagentPlus^®, 99%

Sigma-Aldrich

HATU, 97%

Sigma-Aldrich

(+)-Sodium L-ascorbate, powder, BioReagent, suitable for cell culture

Sigma-Aldrich

Sulfato de magnesio, BioReagent, suitable for cell culture, suitable for insect cell culture

Sigma-Aldrich

Copper(I) bromide, 98%

Sigma-Aldrich

Sodium carbonate decahydrate, puriss. p.a., ≥99.0% (T)

Sigma-Aldrich

Ácido acético, for luminescence, BioUltra, ≥99.5% (GC)

Sigma-Aldrich

Sulfato de magnesio, puriss. p.a., drying agent, anhydrous, ≥98.0% (KT), powder (very fine)

Sigma-Aldrich

Magnesium sulfate solution, for molecular biology, 1.00 M±0.04 M