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Development of an experimental model to study trigeminal nerve-mediated vasodilation on the human forehead.

Cephalalgia : an international journal of headache (2014-01-07)
K Ibrahimi, S Vermeersch, Ahj Danser, C M Villalón, A H van den Meiracker, J de Hoon, A MaassenVanDenBrink
RESUMEN

During migraine, trigeminal sensory nerve terminals release calcitonin gene-related peptide (CGRP), inducing nociception and vasodilation. Applied on the skin, capsaicin activates the transient receptor potential vanilloid type 1 (TRPV1) channel and releases CGRP from sensory nerve terminals, thus increasing dermal blood flow (DBF). Using capsaicin application and electrical stimulation of the forehead skin, a trigeminal nerve-innervated dermatome, we aimed to develop a model to measure trigeminal nerve-mediated vasodilation in humans. Using laser Doppler imaging, forehead DBF responses to application of capsaicin (0.06 mg/ml and 6.0 mg/ml) and saline, with and without iontophoresis, were studied in healthy subjects. The within-subject coefficient of variation (WCV) of repeated DBF measurements was calculated to assess reproducibility. Maximal DBF responses to 6.0 mg/ml capsaicin with and without iontophoresis did not differ (Emax 459 ± 32 and 424 ± 32 arbitrary units (a.u.), WCV 6 ± 4%). In contrast, DBF responses to 0.06 mg/ml capsaicin were significantly larger with than without iontophoresis (Emax 307 ± 60 versus 187 ± 21 a.u., WCV 21 ± 13%). Saline with iontophoresis significantly increased DBF (Emax: 245 ± 26 a.u, WCV 11 ± 8%), while saline application without iontophoresis did not affect DBF. Topical application of capsaicin and electrical stimulation induce reproducible forehead DBF increases and therefore are suitable to study trigeminal nerve-mediated vasodilation in humans.

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Sigma-Aldrich
Capsaicin, ≥95%, from Capsicum sp.
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Sigma-Aldrich
Capsaicin, natural
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USP
Capsaicin, United States Pharmacopeia (USP) Reference Standard
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Sigma-Aldrich
Capsaicin, from Capsicum sp., ≥50% (HPLC)
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Supelco
Capsaicin, analytical standard
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Supelco
Capsaicin, Pharmaceutical Secondary Standard; Certified Reference Material
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Capsaicin, European Pharmacopoeia (EP) Reference Standard
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