Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Gene (2014-02-04)

Gonensin O Bozdag, Alaattin Kaya, Ahmet Koc, Gundula A Noll, Dirk Prüfer, Hüseyin Caglar Karakaya

RESUMEN

Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.

MATERIALES

Referencia del producto

Marca

Descripción del producto

577995

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Nickel, nanopowder, <100 nm avg. part. size, ≥99% trace metals basis

266981

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Nickel, powder, <50 μm, 99.7% trace metals basis

203904

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Nickel, powder, <150 μm, 99.99% trace metals basis

268259

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Nickel, foil, thickness 0.125 mm, ≥99.9%

357634

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268283

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Nickel, powder, <1 μm, 99.8% trace metals basis

357553

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357588

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Nickel, foil, thickness 0.1 mm, 99.98% trace metals basis

267074

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Nickel, rod, diam. 6.35 mm, ≥99.99% trace metals basis

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Nickel, sputtering target, diam. × thickness 2.00 in. × 0.25 in., 99.95% trace metals basis

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Nickel, wire, diam. 0.5 mm, ≥99.99% trace metals basis

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Nickel, mesh, 100x250mm, nominal aperture 0.73mm, wire diameter 0.25mm, 26x26 wires/inch, open area 55%, plain weave mesh, 99%

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357626

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Nickel, wire, diam. 0.5 mm, ≥99.9% trace metals basis

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Nickel, foil, 100x100mm, thickness 0.25mm, annealed, 99%

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Nickel, mesh, 30x30mm, nominal aperture 0.34mm, thickness 0.025mm, wire diameter 0.041mm, 70 wires/inch, open area 80%, electro-formed mesh, 99.9%

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Nickel, foil, 50x50mm, thickness 0.3mm, as rolled, 99.999%

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Nickel, foil, 100x100mm, thickness 0.1mm, annealed, 99%

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Nickel, mesh, 120x120mm, nominal aperture 0.43mm, thickness 0.004mm, wire diameter 0.067mm, 51 wires/inch, open area 75%, electro-formed mesh, 99.9%

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Nickel, wire reel, 100m, diameter 0.025mm, as drawn, 99.98%

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Nickel, foil, 150x150mm, thickness 1.6mm, 99%

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Nickel, mesh, 240x240mm, nominal aperture 0.43mm, thickness 0.004mm, wire diameter 0.067mm, 51 wires/inch, open area 75%, electro-formed mesh, 99.9%

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Nickel, foil, not light tested, 50x50mm, thickness 0.00025mm, temporary acrylic support, 99.95%