SRP2018

GAL4 [(1-147) + E1A (121-223)] from Saccharomyces cerevisiae canine adenovirus

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• Sinónimos: E1A
• UNSPSC Code: 12352202
• NACRES: NA.26

Propiedades

• biological source: Saccharomyces cerevisiae; canine
• recombinant: expressed in E. coli
• assay: ≥70% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~33 kDa
• packaging: pkg of 4 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 100 μg/mL
• technique(s): electrophoretic mobility shift assay: suitable
• color: colorless to clear
• NCBI accession no.: J04368; NM_001184062
• UniProt accession no.: P04386
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: Saccharomyces cerevisiae ... GAL4(855828)

Descripción

Biochem/physiol Actions

The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5`-CGGN5(T/A)N5CCG-3` . A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. The adenovirus E1A protein stimulates transcription of a wide variety of viral and cellular genes. In addition to its trans-activating functions, E1A is also able to modulate progression through the cell cycle, to immortalize primary cells in culture and to induce cellular transformation. The E1A protein binds to the TATA-binding protein, TBP, TAFII55 and TAFII110. Its activating domain has been shown to interact with E2F, ATF-2 and YY1.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable