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Key Documents

R506-05A

Sigma-Aldrich

Rat Lung Fibroblasts: RLF, adult

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

rat lung

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

morphology

fibroblast

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

asthma; tuberculosis; cancer; cardiovascular diseases

shipped in

dry ice

storage temp.

−196°C

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.


RLF from Cell Applications, Inc. have been used to demonstrate that exaggerated (compared to adults) NF-κB activation in neonates in response to hyperoxia is mediated by phosphorylation of IκBα on Tyr42 (Wright, 2009). They were also used to elucidate melatonin effects and signaling pathway (Ben Soussia, 2012) and served as a control in studies investigating epithelial-mesenchimal transition as the source of myofibroblasts responsible for idiopathic pulmonary fibrosis (Willis, 2005; Jain, 2007).

Cell Line Origin

Lungs

Application

Pulmonary function, gene expression, healing & repair process, differentiation, extracellular matrix production, growth factor and cytokine activation, actin reorganization, synthesis of type II collagen, elastin, and proteoglycans, airway remodeling, pharmaceutical development.

Components

Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO

Quality

Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.

Preparation Note

  • 2nd passage, >500,000 cells in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO
  • Can be cultured at least 10 population doublings

Subculture Routine

Please refer to the RLF Culture Protocol.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

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