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GE28-9355-97

Dextrin Sepharose High Performance

Cytiva 28-9355-97, pack of 25 mL

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

dextrin

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 25 mL

manufacturer/tradename

Cytiva 28-9355-97

storage condition

(20% Ehtanol)

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning in place

2-13

working range

>7

capacity

~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium)(Binding capacity is protein dependent. Dynamic binding capacity was tested with MBP-?Sal (MBP2*-paramyosin-?Sal, Mr approx. 70 000, multimer in solution), and MBP-ßGal (MBP2*-beta-galactosidase, Mr approx. 158 000, multimer in solution).)

storage temp.

2-8°C

Categorías relacionadas

General description

Dextrin Sepharose High Perfromance is an affinity media for purification of proteins tagged with Maltose Binding protein (MBP).

Application

Dextrin Sepharose High Performance and provide convenient affinity purification of recombinant Proteins tagged with maltose binding Protein (MBP). This robust and stable Sepharose High Performance based medium enables elution of target Protein in narrow peaks, which minimizes the need for further concentration steps.

Features and Benefits

  • Dextrin Sepharose High Performance is a robust, high resolution medium based on the 34 μm Sepharose High Performance matrix
  • The small, evenly sized beads ensure that MBP-tagged Proteins elute in narrow peaks, thus minimizing the need for further concentration steps
  • Physiological conditions and mild elution preserve target Protein activity
  • Dextrin Sepharose High Performance tolerates all commonly used aqueous buffers and is easily regenerated using 0.5 M NaOH allowing the same column to be used for repeated purifications

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

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Warning

hcodes

Storage Class

3 - Flammable liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Protocolos

This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using GE Healthcare products.

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