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GE17-5163-01

HiTrap® Anx Fast Flow (high sub)

Cytiva 17-5163-01, pack of 5 × 5 mL

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

diethylaminopropyl

description

Ion Exchanger Type (value)

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 17-5163-01

parameter

<20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

4% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

2-14

working range

3-13

suitability

suitable for bioprocess medium

Categorías relacionadas

General description

HiTrap® ANX (high sub) FF is prepacked with ANX (high sub) Sepharose Fast Flow, and is a weak anion exchanger for small scale purification of higher molecular weight protein as well as screening of binding and elutions conditions.

Application

Q, SP, DEAE, and CM Sepharose Fast FLow are based on a robust, 6% highly cross-linked beaded Agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast FLow (high sub) is based on 4% highly cross-linked beaded Agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass Proteins.

The active end of the charged group is the same for DEAE Sepharose Fast FLow and ANX Sepharose Fast FLow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast FLow has a diethylaminoethyl-group bound to the Agarose whilst ANX Sepharose 4 Fast FLow (high sub) has a diethylaminopropyl group attached.

Features and Benefits

  • Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
  • The industry standard for ion exchange chromatography.
  • High fLow rates and good scale-up potential.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  • Predictable scale-up

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

pictograms

Flame

signalword

Warning

hcodes

Storage Class

3 - Flammable liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Protocolos

This page covers the use of Sepharose Fast Flow for purification of proteins.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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