OX-42 Cell Line from murine myeloma

87081803, Reactivity: Anti- CD11, rat

• UNSPSC Code: 41106514

Propiedades

• biological source: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Myeloma: NS0/U
• Reactivity: Anti-CD11, rat
• technique(s): cell culture | mammalian: suitable
• isotype: IgG2a
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse NS0/U x Mouse BALB/c spleen

Cell Line Description

The antibody detects rat CD11. All peritoneal macrophages and 35% of alveolar macrophages react. The antibody affects the function of complement receptor CR3. It immunoprecipitates 3 polypeptides: 160kDa, 130kDa, 95kDa. The antibody also reacts with granulocytes and dendritic cells.

Immunogen

Resident peritoneal cells from (PVG-RT1c x PVG RT1u)F1 and (PVG-RT1c x DA-RT1a)F1 rats

Culture Medium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO₂; 37°C.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.