MM138 Cell Line human

10092321, human skin, Epithelial

• UNSPSC Code: 41106514

Propiedades

• product name: MM138 Cell Line human, 10092321
• biological source: human skin
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human melanoma; from metastatic site - lymph node

Cell Line Description

Metastatic Melanoma (metastatic site: lymph node). Cell Bank Australia reference number: CBA1345.

Culture Medium

RPMI 1640 (with 2 mM L-Glutamine + 25 mM HEPES) + 10% fetal bovine serum (FBS).

Subculture Routine

Optimal split ratio 1:4, MM138 has a relatively slow doubling time of around 60 hours. It does not become confluent and will detach after 6-7 days. If split too hard the doubling time is greatly increased. (Seeding density 0.8 x10⁴ cells/cm²). Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.