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91414

Sigma-Aldrich

Tris buffered saline with Tween® 20

tablet (for 500 mL), pH 7.6, BioUltra, with Tween® 20

Sinónimos:

TBS, TBST

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About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

product name

Tris buffered saline with Tween® 20, BioUltra, tablet (for 500 mL), pH 7.6

product line

BioUltra

form

tablet (for 500 mL)

pH

7.6

solubility

water: 2 tablet/liter, clear, colorless

application(s)

diagnostic assay manufacturing

Categorías relacionadas

Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris buffered saline with Tween 20, pH 7.6 is used as a washing buffer in assays such as enzyme-linked immunosorbent assays.

Reconstitution

1 tablet dissolved in 500 mL of deionized water yields:
0.15 M sodium chloride, 0.050 m TRIS-HCl buffer; 0..05% Tween® 20;
pH 7.6 at 25°C

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Friederike Traunmüller et al.
Journal of immunoassay & immunochemistry, 26(3), 179-188 (2005-07-14)
Immunogenic glycolipids from the cell wall of Mycobacterium tuberculosis are potential capture antigens in enzyme-linked immunosorbent assays (ELISAs) for the serodiagnostis of tuberculosis. Typically, washing steps in ELISAs are performed with buffers containing a detergent. However, Tween-20, the most commonly
T Akimoto et al.
Lupus, 8(9), 761-766 (1999-12-22)
Autoantibodies against prothrombin, including lupus anticoagulant antibodies (LAC), have been identified in patients with systemic lupus erythematosus (SLE) and antiphospholipid syndrome (APS). To identify the epitopes of LAC in patients with SLE and APS, we analyzed B cell epitopes of
Lopa M Das et al.
Autophagy, 15(5), 813-826 (2019-01-22)
Cutaneous inflammation from UV radiation exposure causes epidermal damage, cellular infiltration, and secretion of pro-inflammatory mediators that exacerbate tissue destruction. Recovery is mediated chiefly by anti-inflammatory M2 macrophages that suppress inflammation and augment epidermal regeneration. Vitamin D enables anti-inflammation to
Cesare Orlandi et al.
PloS one, 6(9), e25350-e25350 (2011-10-04)
Ionotropic glutamate α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors are the major mediators of fast synaptic neurotransmission. In this work, we used primary cortical cultures from rats as a model system to study AMPA receptor regulation during in vitro cell maturation and
Bernd Zetsche et al.
Cell, 163(3), 759-771 (2015-10-01)
The microbial adaptive immune system CRISPR mediates defense against foreign genetic elements through two classes of RNA-guided nuclease effectors. Class 1 effectors utilize multi-protein complexes, whereas class 2 effectors rely on single-component effector proteins such as the well-characterized Cas9. Here
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