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Key Documents

14-334-M

Sigma-Aldrich

PRAK Protein, active, 15 µg

Active, N-terminal His6-tagged recombinant, human, full-length PRAK, for use in Kinase Assays.

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.32

biological source

human

Quality Level

100
300

recombinant

expressed in baculovirus infected Sf21 cells

mol wt

Mw 55 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

UniProt accession no.

General description

N-terminal His6-tagged recombinant, human, full-length PRAK
Product Source: expressed by baculovirus in Sf21 insect cells

Biochem/physiol Actions

Protein Target: PRAK
Target Sub-Family: CAMK

Packaging

Also available in 250μg size (2x125μg) - Call for pricing and availability. Please reference catalog number 14-334M when ordering the 250μg size.

Quality

routinely evaluated by phosphorylation of PRAK Substrate Peptide

Physical form

Ni2+/NTA-agarose

Storage and Stability

6 months at -70C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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L New et al.
The EMBO journal, 17(12), 3372-3384 (1998-06-17)
We have identified and cloned a novel serine/ threonine kinase, p38-regulated/activated protein kinase (PRAK). PRAK is a 471 amino acid protein with 20-30% sequence identity to the known MAP kinase-regulated protein kinases RSK1/2/3, MNK1/2 and MAPKAP-K2/3. PRAK was found to
S P Davies et al.
The Biochemical journal, 351(Pt 1), 95-105 (2000-09-22)
The specificities of 28 commercially available compounds reported to be relatively selective inhibitors of particular serine/threonine-specific protein kinases have been examined against a large panel of protein kinases. The compounds KT 5720, Rottlerin and quercetin were found to inhibit many

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