05-1478

Anti-RUNX2 Antibody, clone AS110

clone AS110, from mouse

• Sinónimos: Acute myeloid leukemia 3 protein, CBF-alpha 1, Core-binding factor subunit alpha-1, Oncogene AML-3, Osteoblast-specific transcription factor 2, PEA2-alpha A, PEBP2-alpha A, Polyomavirus enhancer-binding protein 2 alpha A subunit, SL3-3 enhancer factor 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: AS110, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgG2b
• NCBI accession no.: NP_001019801
• UniProt accession no.: Q13950
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... RUNX2(860)

Descripción

General description

RUNX2 (Runt-related transcription Factor) is a transcription factor that binds to its canonical consensus sequence 5’- PYGPYGGT-3’ in a number of promoters and enhancers. They include LCK, T-cell receptor enhancers, and various bone related genes such as osteocalcin, osteopontin, and bone sialoprotein. As a result of this, RUNX2 is involved in osteoblastic differentiation and skeletal morphogenesis. RUNX2 contains a proline/serine/ threonine-rich region at its C-terminus that has shown to be critical for its transcriptional activity.

Specificity

Detects RUNX2

Other species have not been tested.

Immunogen

Epitope: Internal

GST-coupled recombinant protein corresponding to amino acids 311-415 of human RUNX2.

Application

Anti-RUNX2 Antibody, clone AS110 detects level of RUNX2 & has been published & validated for use in WB & IC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Quality

Routinely evaluated by Western Blot.

Western Blot Analysis:
Anti-RUNX2, clone AS110 detected RUNX at 1:1,000 to 1:2,000 dilution in HL-60 cell lysate resolved via SDS-PAGE and transferred to PVDF.

Target description

54-57 kDa

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal in 0.1M Tris-Glycine (pH 7.4) with 150mM NaCl and 0.05% NaN3.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable