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LC/MS/MS Analysis of Vitamins B1 and B6 on Ascentis® Express OH5

Separation of Pyridoxine Hydrochloride (B6), analytical standard; Thiamine Hydrochloride (B1), analytical standard

TLC Analysis of Vitamin C Derivates From Cosmetic Formulation on HPTLC Silica gel 60 F254s

Separation of Placebo; 1 % Vit C derivat; 1 % Active 2; 2 % Vit C Derivat; 2 % Active2; Reference Vitamin C Derivat; Reference Degradation product

Purification from Unclariﬁed Cell Lysate using HisTrap™ FF Crude

This page shows how to purify histidine-tagged recombinant proteins from unclariﬁed cell lysate using HisTrap FF crude from GE Healthcare.

cOmplete™ His-Tag Purification Resin Protocol & Troubleshooting

Anti-HA Affinity Matrix Protocol & Troubleshooting

Tag Removal by Enzymatic Cleavage

This page how to remove histidine tags by enzymatic cleavage using GE Healthcare products.

Sildenafil Citrate USP Method Using HPTLC Plates for the Limit of Imidazole Test

In the present study we demonstrate compliance of silica gel 60 F254 HPTLC plates with USP requirements, and how HPTLC plates are suitable for the Sildenafil citrate-Limit of Imidazole test.

Counterstaining after the Duolink In Situ Protocol

We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.

FSL Construct Protocol

FSL Constructs are compounds based on KODE™ technology designed to label hydrophobic surfaces including living cells with carbohydrates, biotin, fluorophores, or 125I.

Solubilization of Membrane Proteins

This page shows how to solubilize membrane proteins with products from GE Healthcare.

Enzymatic Assay of Phytase (EC 3.1.3.8)

Derived from procedure SSPHYT02. Includes template updates to current SOP specifications and incorporation of notes into the procedure.

Removal of Thrombin and Factor Xa Using HiTrap® Benzamidine FF (High Sub)

This page shows how to remove thrombin and Factor Xa using HiTrap® Benzamidine FF (high sub) from GE Healthcare.

Enzymatic Assay of L-Lactic Dehydrogenase (EC 1.1.1.27)

Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance

Purification of Mouse Monoclonal IgG1 using HiTrap Protein

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap Protein G HP from GE Healthcare for the capture step.

Enzymatic Assay of Amyloglucosidase (EC 3.2.1.3)

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

Packing Tricorn 10/100 columns with MabSelect™ or MabSelect™ SuRe

This page shows how to pack Tricorn 10/100 columns with MabSelect or MabSelect SuRe from GE Healthcare.

HDM-PAMPA with the MultiScreen Permeability Filter Plate

Kit for Creating Hydrophilic PDMS Surfaces

Immunodetection Protocol Using the Immobilon® GO Device

This quick-start guide provides a detailed protocol for the use of the Immobilon® GO device in hands-free, western blot immunodetection.

Protein Determination by the Warburg-Christian Method

Handling Inclusion Bodies in Recombinant Protein Expression

This page how to isolate proteins from inclusion bodies using GE Healthcare products.

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Successful Amplification of Thermally Degraded DNA Using PCRboost™

DNA samples are often exposed to harsh environmental conditions, including heat, humidity, UV or chemicals. Some procedures, such as the extraction of samples from formalin-fixed paraffin embedded tissue samples, subject DNA to multiple extreme conditions.

Aminoglycoside Analysis in Pork Muscle using Molecularly Imprinted Polymer Cleanup and LC/MS/MS Detection

Extraction and quantitative analysis of aminoglycosides in porcine tissue, using molecular imprinted polymer solid phase extraction followed by LC-MS/MS.

Culturing Human Aortic Smooth Muscle Cells

Technical information for working with human aortic smooth muscle cells including thawing, subculturing and cryopreservation.

HPLC Analysis of Barbiturates in Serum on Discovery® C18 after SPE using Discovery® DSC-18Lt

Peptide Resin Loading Protocols

Protocols for loading various peptide resins

Rapid Antibody Concentration with Amicon® Ultra Centrifugal Filters

Purification protocols for immunoglobulins generally include affinity binding to Protein A or G chromatography media, washing unbound proteins, and eluting with a low pH buffer.

Colorimetric Detection of Western blot using 4-Chloro-1-Naphthol Solution Protocol

This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.

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