LC/MS/MS Analysis of Testosterone and Metabolites on Ascentis® Express C18
Materials
standard
Product No.
Description
Pricing
5α-Dihydrotestosterone (DHT) solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®17α-Methyltestosterone solution
1.0 mg/mL in 1,2-dimethoxyethane, ampule of 1 mL, certified reference material, Cerilliant®Testosterone solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)
mobile phase
[A] 5 mM ammonium formate water pH 4.0 formic acid; [B] 0.1% formic acid acetonitrile
gradient
10 to 70% B in 5 min; held at 70% B for 3 min
flow rate
0.6 mL/min
pressure
3698 psi (255 bar)
column temp.
35 °C
detector
MS, ESI(+), TIC, m/z 100-1000
injection
5 μL
sample
1000 ng/mL in 40:60 (water:methanol)
Description
Analysis Note
An Ascentis Expres F5 column provided rapid, baseline resolution of testosterone and metabolites. The highest quality LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany