Subcellular Localization and Activity of TRPM4 in Medial Prefrontal Cortex Layer 2/3.

Subcellular Localization and Activity of TRPM4 in Medial Prefrontal Cortex Layer 2/3.

Frontiers in cellular neuroscience (2018-02-15)

Denise Riquelme, Ian Silva, Ashleigh M Philp, Juan P Huidobro-Toro, Oscar Cerda, James S Trimmer, Elias Leiva-Salcedo

PMID29440991

ABSTRACT

TRPM4 is a Ca

MATERIALS

Product Number

Brand

Product Description

N4014

Sigma-Aldrich

Nystatin, powder, γ-irradiated, BioXtra, suitable for cell culture

G9023

Sigma-Aldrich

Goat serum

MAB377

Sigma-Aldrich

Anti-NeuN Antibody, clone A60, clone A60, Chemicon^®, from mouse

MAB5406

Sigma-Aldrich

Anti-GAD67 Antibody, clone 1G10.2, clone 1G10.2, Chemicon^®, from mouse

L5750

Sigma-Aldrich

Sodium dodecyl sulfate, 92.5-100.5% based on total alkyl sulfate content basis

D5648

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture